Dynamic DNA Assembly by Programmable Hybridization Chain Reaction Mimicking Tubulin

作者全名："Zhang, Li; Fu, Yao; Tong, Yanqiu; Xie, Guoming; Deng, Shixiong"

作者地址："[Zhang, Li; Fu, Yao; Deng, Shixiong] Chongqing Med Univ, Dept Forens Med, Chongqing 400016, Peoples R China; [Zhang, Li; Xie, Guoming] Chongqing Med Univ, Dept Lab Med, Chongqing 400016, Peoples R China; [Tong, Yanqiu] Chongqing Jiaotong Univ, Sch Tourism & Media, Chongqing 400016, Peoples R China"

通信作者："Deng, SX (通讯作者)，Chongqing Med Univ, Dept Forens Med, Chongqing 400016, Peoples R China.; Xie, GM (通讯作者)，Chongqing Med Univ, Dept Lab Med, Chongqing 400016, Peoples R China."

来源：NANO LETTERS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001167199200001

JCR分区：Q1

影响因子：9.6

年份：2024

卷号：24

期号：8

开始页：2603

结束页：2610

文献类型：Article

关键词：hybridization chain reaction; dynamic self-assembly; conformational switching; metastable domain; tubulin

摘要："Dynamic biological structures involve the continual turnover of molecules within supramolecular assemblies such as tubulin. Inspired by dynamic biology self-organizing systems, we build an artificial dynamic structure based on DNA nanotechnology through a nonequilibrium chemical system. Herein, a metastable domain (MD), essentially a stem-loop structure, was introduced into DNA hairpins within hybridization chain reaction (HCR), thereby imparting dynamic activity to the DNA polymers. Hairpins with MD thermodynamically assemble to a high-energy polymer in the presence of trigger strands. The polymer can relax back to the stable unassembled state once the invader is added and finally relax to the activated hairpin by an anti-invader. Reversible assembly/disassembly of the HCR is achieved through invader/anti-invader cycles. We accomplished kinetic modulation, reversible conformational switching, cascading regulation, and enzyme activity control through the MD-HCR. We believe that the design of the MD-HCR could inspire the development of autonomous biological functions within artificial systems."

基金机构：National Key Research and Development Program of China [cstc2021jcyj-msxmX0485]; Natural Science Foundation of Chongqing Province [2022YFC2603803]; National Key R&D Program of China [19YJA860022]; Humanities & Social Sciences of the Ministry of Education of the People's Republic of China

基金资助正文："This research work was financially supported by the Natural Science Foundation of Chongqing Province (cstc2021jcyj-msxmX0485), the National Key R&D Program of China (2022YFC2603803), and the Humanities & Social Sciences of the Ministry of Education of the People's Republic of China (19YJA860022)."