Microarray analysis identifies lncFirre as a potential regulator of obesity-related acute lung injury

作者全名："Tang, Wenjing; Wu, Siqi; Tang, Yin; Ma, Jingyue; Ao, Yichan; Liu, Ling; Wei, Ke"

作者地址："[Tang, Wenjing; Wu, Siqi; Tang, Yin; Ma, Jingyue; Ao, Yichan; Liu, Ling; Wei, Ke] Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Liu, L; Wei, K (通讯作者)，Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：LIFE SCIENCES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001178238100001

JCR分区：Q1

影响因子：6.1

年份：2024

卷号：340

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Obesity; Acute lung injury; lncFirre; Palmitate; Inflammation

摘要："Aims: The inflammatory response in acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is heightened in obesity. The aim of this study was to investigate whether lncRNAs are involved in the effects of obesity on acute lung injury and to find possible effector lncRNAs. Main methods: Microarray analysis was used to assess the transcriptional profiles of lncRNAs and mRNAs in lung tissues from normal (CON), high-fat diet induced obese (DIO), and obese ALI mice (DIO-ALI). GO and KEGG analyses were employed to explore the biological functions of differentially expressed genes. A lncRNA-mRNA co-expression network was constructed to identify specific lncRNA. Lung tissues and peripheral blood samples from patients with obesity and healthy lean donors were utilized to confirm the expression characteristics of lncFirre through qRT-PCR. lncFirre was knocked down in MH-S macrophages to explore its function. ELISA and Griess reagent kit were used to detect PGE2 and NO. Flow cytometry was used to detect macrophages polarization. Key findings: There were 475 lncRNAs and 404 mRNAs differentially expressed between DIO and CON, while 1348 lncRNAs and 1349 mRNAs between DIO-ALI and DIO. Obesity increased lncFirre expression in both mice and patients, and PA elevated lncFirre in MH-S. PA exacerbated the inflammation and proinflammatory polarization of MH-S induced by LPS. LncFirre knockdown inhibited the secretion of PGE2 and NO, M1 differentiation while promoted the M2 differentiation in PA and LPS co-challenged MH-S. Significance: Interfering with lncFirre effectively inhibit inflammation in MH-S, lncFirre can serve as a promising target for treating obesity-related ALI."

基金机构：Chongqing Science AMP; Technology Bureau [CSTC2019jscx-msxmX0214]; Chongqing Health Commission for the advanced talent studio

基金资助正文：This study was supported by grants from Chongqing Science & Technology Bureau (Project no. CSTC2019jscx-msxmX0214) and from Chongqing Health Commission for the advanced talent studio. The content is solely the responsibility of the authors and does not represent the official views of the Chongqing Science & Technology Bureau.