Paper The tsRNAs (tRFdb-3013a/b) serve as novel biomarkers for colon adenocarcinomas

作者全名："Tan, Lihong; Wu, Xiaoling; Tang, Zhurong; Chen, Huan; Cao, Weiguo; Wen, Chunjie; Zou, Guojun; Zou, Hecun"

作者地址："[Tan, Lihong; Wu, Xiaoling] Chongqing Med & Pharmaceut Coll, Chongqing 401331, Peoples R China; [Chen, Huan; Zou, Guojun] Yueyang Hosp Tradit Chinese Med, Yueyang 414000, Peoples R China; [Tang, Zhurong; Chen, Huan; Cao, Weiguo; Wen, Chunjie; Zou, Hecun] Chongqing Med Univ, Chongqing 400016, Peoples R China"

通信作者："Zou, GJ (通讯作者)，Yueyang Hosp Tradit Chinese Med, Yueyang 414000, Peoples R China.; Zou, HC (通讯作者)，Chongqing Med Univ, Chongqing 400016, Peoples R China."

来源：AGING-US

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001188188200006

JCR分区：Q2

影响因子：3.9

年份：2024

卷号：16

期号：5

开始页：4299

结束页：4326

文献类型：Article

关键词：tsRNA; tRFdb-3013a; tRFdb-3013b; colon adenocarcinomas; ST3GAL1

摘要："The tsRNAs (tRNA-derived small RNAs) are a novel class of small non-coding RNAs derived from transfer-RNAs. Colon adenocarcinoma (COAD) is the most malignant intestinal tumor. This study focused on the identification and characterization of tsRNA biomarkers in colon adenocarcinomas. Data processing and bioinformatic analyses were performed with the packages of R and Python software. The cell proliferation, migration and invasion abilities were determined by CCK-8 and transwell assays. Luciferase reporter assay was used to test the binding of tsRNA with its target genes. With computational methods, we identified the tRNA fragments profiles within COAD datasets, and discriminated forty-two differentially expressed tsRNAs between paired colon adenocarcinomas and non -tumor controls. Among the fragments derived from the 3 ' end of tRNA-HisGUG (a histidyl-transfer-RNA), tRFdb-3013a and tRFdb-3013b (tRFdb-3013a/b) were notably decreased in colon and rectum adenocarcinomas, especially, tRFdb-3013a/b might tend to be down-regulated in patients with lymphatic or vascular invasion present. The clinical survival of colorectal adenocarcinoma patients with low tRFdb-3013a/b expression was significantly worse than that of high expression patients. In colon adenocarcinoma cells, tRFdb-3013a could have inhibited cell proliferations, and reduced cell migration and invasion abilities. The enrichment analyses showed that most of tRFdb-3013a correlated-genes were enriched in the extracellular matrix associated GO terms, phagosome pathway, and a GSEA molecular signature pathway. Additionally, the 3 ' UTR of ST3GAL1 mRNA was predicted to contain the binding site of tRFdb3013a/b, tRFdb-3013a/b might directly target and regulate ST3GAL1 expression in colon adenocarcinomas. These results suggested that tRFdb-3013a/b might serve as novel biomarkers for diagnosis and prognosis of colon adenocarcinomas, and act a key player in the progression of colon adenocarcinomas."

基金机构："Natural Science Foundation of Chongqing, China [cstc2021jcyj- msxmX0302]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN202200430]; National Natural Science Foundation of China [82003854]; Program for Youth Innovation in Future Medicine from Chongqing Medical University [W0066]; Supercomputing Center of Chongqing Medical University"

基金资助正文："This work was sponsored by Natural Science Foundation of Chongqing, China (cstc2021jcyj- msxmX0302) , and supported by the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJQN202200430) . This work was also supported by grants from the National Natural Science Foundation of China (No. 82003854) , and the Program for Youth Innovation in Future Medicine from Chongqing Medical University (W0066) . The computing work was partly supported by the Supercomputing Center of Chongqing Medical University."