Surface-Activated Ti₃C₂T_x Adsorption of Acetylene Black Coupled with Polyaniline as a Signal Tag for the Detection of the ESAT-6 Antigen of <i>Mycobacterium tuberculosis</i>

作者全名："Zuo, Jianli; Yuan, Yonghua; Qing, Min; Chen, Yuhan; Huang, He; Zhou, Jiaxu; Bai, Lijuan; Liang, Hongwen"

作者地址："[Zuo, Jianli; Yuan, Yonghua; Qing, Min; Chen, Yuhan; Huang, He; Zhou, Jiaxu; Bai, Lijuan; Liang, Hongwen] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Zuo, Jianli; Yuan, Yonghua; Qing, Min; Chen, Yuhan; Huang, He; Zhou, Jiaxu; Bai, Lijuan; Liang, Hongwen] Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ; Liang, HW (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China.; Bai, LJ; Liang, HW (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing 400016, Peoples R China."

来源：ACS APPLIED MATERIALS & INTERFACES

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001195097400001

JCR分区：Q1

影响因子：8.3

年份：2024

卷号：16

期号：14

开始页：17300

结束页：17312

文献类型：Article

关键词：electrochemical aptasensor; signal tag; nanoflower; Ti3C2Tx; ESAT-6 antigen

摘要："Early secretory antigenic target-6 (ESAT-6) is regarded as the most immunogenic protein produced by Mycobacterium tuberculosis, whose detection is of great clinical significance for tuberculosis diagnosis. However, the detection of the ESAT-6 antigen has been hampered by the expensive cost and complex experimental procedures, resulting in low sensitivity. Herein, we developed a titanium carbide (Ti3C2Tx)-based aptasensor for ESAT-6 detection utilizing a triple-signal amplification strategy. First, acetylene black (AB) was immobilized on Ti3C2Tx through a cross-linking reaction to form the Ti3C2Tx-AB-PAn nanocomposite. Meanwhile, AB served as a conductive bridge, and Ti3C2Tx can synergistically promote the electron transfer of PAn. Ti3C2Tx-AB-PAn exhibited outstanding conductivity, high electrochemical signals, and abundant sites for the loading of ESAT-6 binding aptamer II (EBA II) to form a novel signal tag. Second, N-CNTs were adsorbed on NiMn layered double hydride (NiMn LDH) nanoflowers to obtain NiMn LDH/N-CNTs, exhibiting excellent conductivity and preeminent stability to be used as electrode modification materials. Third, the biotinylated EBA (EBA I) was immobilized onto a streptavidin-coated sensing interface, forming an amplification platform for further signal enhancement. More importantly, as a result of the synergistic effect of the triple-signal amplification platform, the aptasensor exhibited a wide detection linear range from 10 fg mL(-1) to 100 ng mL(-1) and a detection limit of 4.07 fg mL(-1) for ESAT-6. We envision that our aptasensor provides a way for the detection of ESAT-6 to assist in the diagnosis of tuberculosis."

基金机构："National Natural Science Foundation of China [82072378, 22007024, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019); The 2023 Graduate Research and Innovation Project of Chongqing, China [CYB23218]; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2021BSH04]; CQMU Program for Youth Innovation in Future Medicine, China [172020320220090 W0072]; Natural Science Foundation of Chongqing, China [CSTB2022NSCQ-BHX0006, CSTB2023NSCQ-MSX0314]"

基金资助正文："This work is supported by the National Natural Science Foundation of China (82072378, 22007024, 81601856); Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328); Ba Yu Scholar Program, China (YS2019020); Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067); Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019); 2023 Graduate Research and Innovation Project of Chongqing, China (CYB23218); Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2021BSH04); CQMU Program for Youth Innovation in Future Medicine, China (172020320220090 W0072); and Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-BHX0006, CSTB2023NSCQ-MSX0314)."