Prognostic effect of DNA methylation of BTG2 gene in Chinese hepatocellular carcinoma

作者全名：Ma, Jungang; Chen, Zhuo; Liu, Shuixia; Chen, Chuan; Guan, Wei; Geng, Mingying; Xiao, He; Mao, Bijing; Wang, Bin

作者地址：[Ma, Jungang; Chen, Zhuo; Chen, Chuan; Guan, Wei; Geng, Mingying; Xiao, He] Army Med Univ, Daping Hosp, Dept Oncol, Chongqing 400042, Peoples R China; [Liu, Shuixia] Army Med Univ, Daping Hosp, Dept Ophthalmol, Chongqing 400042, Peoples R China; [Mao, Bijing] Chongqing Med Univ, Affiliated Hosp 3, Dept Oncol, 1 Shuanghu Branch Rd, Chongqing 401120, Peoples R China; [Wang, Bin] Chongqing Univ Technol, Affiliated Cent Hosp, Peoples Hosp Chongqing 7, Dept Oncol, Lijiatuo St, Chongqing 400054, Peoples R China

通信作者：Mao, BJ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Oncol, 1 Shuanghu Branch Rd, Chongqing 401120, Peoples R China.; Wang, B (通讯作者)，Chongqing Univ Technol, Affiliated Cent Hosp, Peoples Hosp Chongqing 7, Dept Oncol, Lijiatuo St, Chongqing 400054, Peoples R China.

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001215471300001

JCR分区：Q1

影响因子：3.4

年份：2024

卷号：10

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：Hepatocellular carcinoma; DNA methylation; B-cell translocation gene 2; Prognosis

摘要：Background: This study aims to develop a prognostic model for overall survival based on potential methylation sites within B-cell translocation gene 2 ( BTG2 ) in Chinese patients with hepatocellular carcinoma (HCC). Methods: This is a retrospective study. The beta values of nine CpG sites and RSEM normalized count values of BTG2 gene were extracted from the Cancer Genome Atlas -Liver Hepatocellular Carcinoma (TCGA-LIHC) (TCGA-LIHC) dataset, with the beta value representing the methylation level by indicating the ratio of the intensity of the methylated bead type to the combined locus intensity. Pyrosequencing was performed to determine the range of methylation values surrounding cg01798157 site in BTG2 gene. A weighted linear model was developed to predict the overall survival (OS). Results: The beta value of cg01798157 was significantly negatively associated with the mRNA expression of BTG2 in the TCGA-LIHC dataset (Spearman 's rho = -0.5306, P = 2.27 x 10 -27 ). The methylation level of cg01798157 was significantly associated with OS in the cohort of 51 Chinese HCC patients (Hazard ratio = 0.597, 95% CI: 0.434 -0.820, P = 0.001). Multivariate Cox regression analysis identified methylation level of cg01798157, cirrhosis, and microvascular invasion as independent prognostic factors. The prognostic efficiency of death risk score was superior to that of cirrhosis or microvascular invasion alone. Conclusions: The methylation level of cg01798157 in BTG2 may be an epigenetic biomarker in Chinese patients with resectable HCC.

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