Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence

作者全名：Zhao, Feng; Chen, Anli; Wu, Xiaotian; Deng, Xiangyu; Yang, Jiali; Xue, Jianjiang

作者地址：[Zhao, Feng; Xue, Jianjiang] Chongqing Med Univ, Sch Publ Hlth, Dept Hlth Lab Technol, 55 Daxuecheng Middle Rd, Chongqing 400016, Peoples R China; [Chen, Anli; Wu, Xiaotian; Deng, Xiangyu; Xue, Jianjiang] Chongqing Med Univ, Dept Clin Lab, Univ Town Hosp, Chongqing 401331, Peoples R China; [Yang, Jiali] Chongqing Gen Hosp, Inst Hepatopancreatobiliary Surg, Chongqing 401147, Peoples R China

通信作者：Xue, JJ (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Dept Hlth Lab Technol, 55 Daxuecheng Middle Rd, Chongqing 400016, Peoples R China.; Xue, JJ (通讯作者)，Chongqing Med Univ, Dept Clin Lab, Univ Town Hosp, Chongqing 401331, Peoples R China.; Yang, JL (通讯作者)，Chongqing Gen Hosp, Inst Hepatopancreatobiliary Surg, Chongqing 401147, Peoples R China.

来源：BMC CANCER

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001217345900006

JCR分区：Q2

影响因子：3.4

年份：2024

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Pancreatic cancer; Tumor microorganisms; Gut microbes; Tumor markers

摘要：Background Pancreatic cancer is the foremost contributor to cancer-related deaths globally, and its prevalence continues to rise annually. Nevertheless, the underlying mechanisms behind its development remain unclear and necessitate comprehensive investigation. Methods In this study, a total of 29 fresh stool samples were collected from patients diagnosed with pancreatic cancer. The gut microbial data of healthy controls were obtained from the SRA database (SRA data number: SRP150089). Additionally, 28 serum samples and diseased tissues were collected from 14 patients with confirmed pancreatic cancer and 14 patients with chronic pancreatitis. Informed consent was obtained from both groups of patients. Microbial sequencing was performed using 16s rRNA. Results The results showed that compared with healthy controls, the species abundance index of intestinal flora in patients with pancreatic cancer was increased (P < 0.05), and the number of beneficial bacteria at the genus level was reduced (P < 0.05). Compared with patients with chronic pancreatitis, the expression levels of CA242 and CA199 in the serum of patients with pancreatic cancer were increased (P < 0.05). The bacterial richness index of tumor microorganisms in patients with pancreatic cancer increased, while the diversity index decreased(P < 0.05). Furthermore, there was a change in the species composition at the genus level. Additionally, the expression level of CA242 was found to be significantly positively correlated with the relative abundance of Acinetobacter(P < 0.05). Conclusion Over all, the expression levels of serum tumor markers CA242 and CA19-9 in patients with pancreatic cancer are increased, while the beneficial bacteria in the intestine and tumor microenvironment are reduced and pathogenic bacteria are increased. Acinetobacter is a specific bacterial genus highly expressed in pancreatic cancer tissue.

基金机构：Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) of China

基金资助正文：We express our gratitude to Chengzhi Chen and Xianqing Cao for their technical assistance, Jingfu Qiu for the contribution to previous studies that facilitated this present work, and Xianquan Zhuang for editing the manuscript.