Potential novel <i>Colpodella </i>spp. (phylum Apicomplexa) and high prevalence of Colpodella spp. in goat-attached <i>Haemaphysalis longicornis</i> ticks in Shandong province, China

作者全名：Qi, Yong; Wang, Junhu; Lu, Nianhong; Qi, Xin; Yang, Chaoyue; Liu, Bing; Lu, Yongfeng; Gu, Yuan; Tan, Weilong; Zhu, Changqiang; Ai, Lele; Rao, Jixian; Mao, Yingqing; Yi, Haiming; Li, Yuexi; Yue, Ming

作者地址：[Qi, Yong; Wang, Junhu; Lu, Nianhong; Tan, Weilong; Zhu, Changqiang; Ai, Lele; Rao, Jixian; Mao, Yingqing; Yi, Haiming; Li, Yuexi] Huadong Res Inst Med & Biotech, 293 Zhongshandonglu, Nanjing 210002, Jiangsu, Peoples R China; [Qi, Xin] Second Peoples Hosp Yiyuan Cty, Zibo 256100, Shandong, Peoples R China; [Yang, Chaoyue] Army Med Univ, Chongqing 400038, Peoples R China; [Liu, Bing] Rocket Force Characterist Med Ctr, Dept Dis Control & Prevent, 16 Xinjiekouwai St, Beijing 100088, Peoples R China; [Lu, Yongfeng] Adm Drug & Instrument Supervis & Inspection PLAJLS, Beijing 100071, Peoples R China; [Gu, Yuan] Nanjing Univ, Jinling Hosp, Outpatient Dept 9, Med Sch, Nanjing 210002, Jiangsu, Peoples R China; [Yue, Ming] Nanjing Med Univ, Dept Infect Dis, Affiliated Hosp 1, Nanjing, Peoples R China

通信作者：Yue, M (通讯作者)，Nanjing Med Univ, Dept Infect Dis, Affiliated Hosp 1, Nanjing, Peoples R China.

来源：TICKS AND TICK-BORNE DISEASES

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001218669300001

JCR分区：Q1

影响因子：3.1

年份：2024

卷号：15

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：Tick-borne diseases; Theileria luwenshuni; Haemaphysalis longicornis; Colpodella; Molecular epidemiology

摘要：Tick-borne Apicomplexan parasites pose a significant threat to both public health and animal husbandry. Identifying potential pathogenic parasites and gathering their epidemiological data are essential for prospectively preventing and controlling infections. In the present study, genomic DNA of ticks collected from two goat flocks (Goatflock1 and Goatflock2) and one dog group (Doggroup) were extracted and the 18S rRNA gene of Babesia/Theileria/Colpodella spp. was amplified by PCR and sequenced. Phylogenetic analysis was conducted based on the obtained sequences. The differences in pathogen positive rates between ticks of different groups were statistically analyzed using the Chi-square or continuity-adjusted Chi-square test. As a result, two pathogenic Theileria (T.) luwenshuni genotypes, one novel pathogenic Colpodella sp. HLJ genotype, and two potential novel Colpodella spp. (referred to as Colpodella sp. struthionis and Colpodella sp. yiyuansis in this study) were identified in the Haemaphysalis (H.) longicornis ticks. Ticks of Goatflock2 had a significantly higher positive rate of Colpodella spp. than those from Goatflock1 (chi 2=92.10; P = 8.2 x 10(-22)) and Doggroup (chi 2=42.34; P = 7.7 x 10(-11)), and a significantly higher positive rate of T. luwenshuni than Doggroup (chi 2=5.38; P = 0.02). However, the positive rates of T. luwenshuni between Goatflock1 and Goatflock2 were not significantly different (chi 2=2.02; P = 0.16), and so as the positive rates of both pathogens between Goatflock1 and Doggroup groups (P > 0.05). For either Colpodella spp. or T. luwenshuni, no significant difference was found in prevalence between male and female ticks. These findings underscore the potential importance of Colpodella spp. in domestic animal-attached ticks, as our study revealed two novel Colpodella spp. and identified Colpodella spp. in H. longicornis for the first time. The study also sheds light on goats' potential roles in the transmission of Colpodella spp. to ticks and provides crucial epidemiological data of pathogenic Theileria and Colpodella. These data may help physicians, veterinarians, and public health officers prepare suitable detection and treatment methods and develop prevention and control strategies.

基金机构：Medical Science and Technology Projects [JK2023002, JK2023003, JK2023004]; National Natural Science Foundation of China [82273691]; Open Research Fund Program of the State Key Laboratory of Pathogen and Biosecurity [SKLPBS2137]

基金资助正文：This study was funded by Medical Science and Technology Projects (JK2023002, JK2023003, and JK2023004) , National Natural Science Foundation of China (82273691) , and Open Research Fund Program of the State Key Laboratory of Pathogen and Biosecurity (No. SKLPBS2137) .