Ultrasensitive serological digoxin fluorescence sensor based on cascade amplification of entropy-driven DNA nanomachine and proximity ligation assay

作者全名：Li, Keyu; Xiao, Peng; Wang, Chuning; Zhang, Zhilun; Feng, Jiaxi; Cai, Yijia; Xiang, Yixiao; Bai, Xuelu; Zuo, Guowei; Han, Baoru

作者地址：[Li, Keyu; Xiao, Peng; Feng, Jiaxi; Cai, Yijia; Xiang, Yixiao; Bai, Xuelu; Zuo, Guowei] Chongqing Med Univ, Chinese Minist Educ, Dept Lab Med, Key Lab Diag Med Designated, Chongqing 400016, Peoples R China; [Wang, Chuning] Xinjiang Univ, Coll Life Sci & Technol, Urumqi 830046, Peoples R China; [Zhang, Zhilun] Chongqing Med Univ, Yongchuan Hosp, Dept Oncol, Chongqing, Peoples R China; [Han, Baoru] Chongqing Med Univ, Med Data Sci Acad, Coll Med Informat, Chongqing 400016, Peoples R China

通信作者：Zuo, GW (通讯作者)，Chongqing Med Univ, Chinese Minist Educ, Dept Lab Med, Key Lab Diag Med Designated, Chongqing 400016, Peoples R China.

来源：MICROCHEMICAL JOURNAL

ESI学科分类：CHEMISTRY

WOS号：WOS:001226886900001

JCR分区：Q1

影响因子：4.9

年份：2024

卷号：200

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Digoxin; Aptamer; DNA nanomachine; Toehold -mediated strand displacement; Proximity ligation assay; Fluorescence

摘要：A nanobiosensor was prepared by DNA nanomachine based on proximity ligation assay (PLA) for the detection of digoxin (DIG) in serum. In this sensor the DNA nanomachine releases a large number of signal probes (SP) after addition of DIG, which participate in PLA as the connector strand, initiating real-time quantitative polymerase chain reaction (qPCR) and producing fluorescence signals. For the benefit of clinical therapeutics, we determined the detection range of 0.1 similar to 5 ng/mL. Under the optimal experimental conditions, the cycle threshold (Ct) value showed a good linear relationship with DIG concentrations, and the detection limit was as low as 0.06 ng/mL (3 sigma). This method achieved the ultrasensitive detection of DIG by signal amplification and was successfully used for the detection of DIG in real serum, which has a good potential for application in the field of therapeutic drug monitoring.

基金机构：Intelligent Medical Project of Chongqing Medical University, China [ZHYX202206]

基金资助正文：This work was financially supported by the Intelligent Medical Project of Chongqing Medical University, China (No. ZHYX202206) .