Hybridization alters red deer gut microbiome and metabolites

作者全名：Wei, Limin; Zeng, Bo; Li, Bo; Guo, Wei; Mu, Zhenqiang; Gan, Yunong; Li, Yanhong

作者地址：[Wei, Limin; Mu, Zhenqiang] Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing, Peoples R China; [Wei, Limin] Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China; [Zeng, Bo] Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key L, Chengdu, Peoples R China; [Li, Bo] Aba Teachers Univ, Coll Resources & Environm, Aba, Peoples R China; [Guo, Wei; Gan, Yunong] Chengdu Med Coll, Sch Lab Med, Chengdu, Peoples R China; [Li, Yanhong] Guizhou Med Univ, Key Lab Endem & Ethn Dis, Minist Educ, Guiyang, Guizhou, Peoples R China; [Li, Yanhong] Guizhou Med Univ, Key Lab Med Mol Biol Guizhou Prov, Guiyang, Guizhou, Peoples R China; [Li, Yanhong] Guizhou Med Univ, Collaborat Innovat Ctr Prevent & Control Endem & E, Guiyang, Guizhou, Peoples R China

通信作者：Zeng, B (通讯作者)，Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key L, Chengdu, Peoples R China.; Li, YH (通讯作者)，Guizhou Med Univ, Key Lab Endem & Ethn Dis, Minist Educ, Guiyang, Guizhou, Peoples R China.; Li, YH (通讯作者)，Guizhou Med Univ, Key Lab Med Mol Biol Guizhou Prov, Guiyang, Guizhou, Peoples R China.; Li, YH (通讯作者)，Guizhou Med Univ, Collaborat Innovat Ctr Prevent & Control Endem & E, Guiyang, Guizhou, Peoples R China.

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001229531500001

JCR分区：Q2

影响因子：4

年份：2024

卷号：15

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：red deer; hybridization; gut microbiome; pilose antler; multi-omics technologies

摘要：The host genes play a crucial role in shaping the composition and structure of the gut microbiome. Red deer is listed as an endangered species by the International Union for the Conservation of Nature, and its pilose antlers have good medicinal value. Hybridization can lead to heterosis, resulting in increased pilose antler production and growth performance in hybrid deer. However, the role of the gut microbiome in hybrid deer remains largely unknown. In this study, alpha and beta diversity analysis showed that hybridization altered the composition and structure of the gut microbiome of the offspring, with the composition and structure of the hybrid offspring being more similar to those of the paternal parents. Interestingly, the LefSe differential analysis showed that there were some significantly enriched gut microbiome in the paternal parents (such as g_Prevotellaceae UCG-003, f_Bacteroidales RF16 group; Ambiguous_taxa, etc.) and the maternal parents (including g_Alistipes, g_Anaerosporobacter, etc.), which remained significantly enriched in the hybrid offspring. Additionally, the hybrid offspring exhibited a significant advantage over the parental strains, particularly in taxa that can produce short-chain fatty acids, such as g_Prevotellaceae UCG-003, g_Roseburia, g_Succinivibrio, and g_Lachnospiraceae UCG-006. Similar to bacterial transmission, metagenomic analysis showed that some signaling pathways related to pilose antler growth ("Wnt signaling pathway," "PI3K Akt signaling pathway," "MAPK signaling pathway") were also enriched in hybrid red deer after hybridization. Furthermore, metabolomic analysis revealed that compared with the paternal and maternal parents, the hybrid offspring exhibited significant enrichment in metabolites related to "Steroid hormone biosynthesis," "Tryptophan metabolism," "Valine, leucine and isoleucine metabolism," and "Vitamin B metabolism." Notably, the metagenomic analysis also showed that these metabolic pathways were significantly enriched in hybrid deer. Finally, a correlation analysis between the gut microbiome and metabolites revealed a significant positive correlation between the enriched taxa in hybrid deer, including the Bacteroidales RF16 group, Prevotellaceae, and Succinivibrio, and metabolites, such as 7 alpha-hydroxytestosterone, L-kynurenine, indole, L-isoleucine, and riboflavin. The study contributes valuable data toward understanding the role of the gut microbiome from red deer in hybridization and provides reference data for further screening potential probiotics and performing microbial-assisted breeding that promotes the growth of red deer pilose antlers and bodies, development, and immunity.

基金机构：Chongqing Municipal Education Commission10.13039/501100007957

基金资助正文：The authors gratefully acknowledge the staff of the red deer farm in Xinjiang.