Identification of circRNA Expression Profile and Potential Systemic Immune Imbalance Modulation in Premature Rupture of Membranes

作者全名：Huang, Dongni; Ran, Yuxin; Chen, Ruixin; He, Jie; Yin, Nanlin; Qi, Hongbo

作者地址：[Huang, Dongni; Ran, Yuxin; He, Jie; Qi, Hongbo] Chongqing Med Univ, Women & Childrens Hosp, Chongqing Hlth Ctr Women & Children, Chongqing, Peoples R China; [Huang, Dongni; Ran, Yuxin; He, Jie; Qi, Hongbo] Chongqing Med Univ, Chongqing Key Lab Maternal & Fetal Med, Chongqing, Peoples R China; [Chen, Ruixin] Sichuan Univ, West China Hosp 2, Dept Gynecol & Obstet, Chengdu, Peoples R China; [Yin, Nanlin] Chongqing Med Univ, Affiliated Hosp 1, Ctr Reprod Med, Chongqing, Peoples R China

通信作者：Qi, HB (通讯作者)，Chongqing Med Univ, Women & Childrens Hosp, Chongqing Hlth Ctr Women & Children, Chongqing, Peoples R China.; Qi, HB (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Maternal & Fetal Med, Chongqing, Peoples R China.; Yin, NL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Ctr Reprod Med, Chongqing, Peoples R China.

来源：ANALYTICAL CELLULAR PATHOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001236779600001

JCR分区：Q2

影响因子：2.6

年份：2024

卷号：2024

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要：Premature rupture of membrane (PROM) refers to the rupture of membranes before the onset of labor which increases the risk of perinatal morbidity and mortality. Recently, circular RNAs (circRNAs) have emerged as promising regulators of diverse diseases. However, the circRNA expression profiles and potential circRNA-miRNA-mRNA regulatory mechanisms in PROM remain enigmatic. In this study, we displayed the expression profiles of circRNAs and mRNAs in plasma and fetal membranes of PROM and normal control (NC) groups based on circRNA microarray, the Gene Expression Omnibus database, and NCBI's Sequence Read Archive. A total of 1,459 differentially expressed circRNAs (DECs) in PROM were identified, with 406 upregulated and 1,053 downregulated. Then, we constructed the circRNA-miRNA-mRNA network in PROM, encompassing 22 circRNA-miRNA pairs and 128 miRNA-mRNA pairs. Based on the analysis of gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene set enrichment analysis (GSEA), DECs were implicated in immune-related pathways, with certain alterations persisting even postpartum. Notably, 11 host genes shared by DECs of fetal membrane tissue and prenatal plasma in PROM were significantly implicated in inflammatory processes and extracellular matrix regulation. Our results suggest that structurally stable circRNAs may predispose to PROM by mediating systemic immune imbalances, including peripheral leukocyte disorganization, local immune imbalance at the maternal-fetal interface, and local collagen disruption. This is the first time to decipher a landscape on circRNAs of PROM, reveals the pathogenic cause of PROM from the perspective of circRNA, and opens up a new direction for the diagnosis and treatment of PROM.

基金机构：Chongqing Municipal Health Commission; Chongqing Science and Technology Commission [2023GGXM005]; Sichuan Science and Technology Program [2023YFQ0005]; Key Research Program of Chongqing Science and Technology Bureau [CSTB2022TIAD-KPX0156]; China Postdoctoral Science Foundation [2023M740447]; National Key Clinical Specialty Construction Project (Obstetrics and Gynecology); Ministry of Education, China; State Administration of Foreign Experts Affairs, China

基金资助正文：This work was supported by grants from the Chongqing Municipal Health Commission and the Chongqing Science and Technology Commission (No. 2023GGXM005), Sichuan Science and Technology Program (No. 2023YFQ0005), the Key Research Program of Chongqing Science and Technology Bureau (No. CSTB2022TIAD-KPX0156), the China Postdoctoral Science Foundation (No. 2023M740447), and the National Key Clinical Specialty Construction Project (Obstetrics and Gynecology). The authors thank E Gong of the Department of Obstetrics and all the research staff of the Department of Scientific Research and Education, Women and Children's Hospital of Chongqing Medical University (Chongqing Health Center for Women and Children). The authors would like to appreciate the support from the "111 program" of Ministry of Education, China and State Administration of Foreign Experts Affairs, China.