CSTF3 contributes to platinum resistance in ovarian cancer through alternative polyadenylation of lncRNA NEAT1 and generating the short isoform NEAT1_1

作者全名：Luo, Xin; Wei, Qinglv; Jiang, Xiaoyan; Chen, Ningxuan; Zuo, Xinzhao; Zhao, Hongyan; Liu, Yujiao; Liu, Xiaoyi; Xie, Lingcui; Yang, Yu; Liu, Tao; Yi, Ping; Xu, Jing

作者地址：[Luo, Xin; Jiang, Xiaoyan; Chen, Ningxuan; Zuo, Xinzhao; Liu, Yujiao; Liu, Xiaoyi; Xie, Lingcui; Yang, Yu; Liu, Tao; Yi, Ping; Xu, Jing] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 3, Chongqing, Peoples R China; [Wei, Qinglv] Chongqing Med Univ, Chongqing Key Lab Child Infect & Immun, Childrens Hosp, Chongqing, Peoples R China; [Zhao, Hongyan] Hubei Univ Med, Sch Basic Med, Shiyan, Hubei, Peoples R China

通信作者：Liu, T; Yi, P; Xu, J (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 3, Chongqing, Peoples R China.

来源：CELL DEATH & DISEASE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001251451800004

JCR分区：Q1

影响因子：8.1

年份：2024

卷号：15

期号：6

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要：Platinum-based chemotherapy is the standard postoperative adjuvant treatment for ovarian cancer (OC). Despite the initial response to chemotherapy, 85% of advanced OC patients will have recurrent disease. Relapsed disease and platinum resistance are the major causes of death in OC patients. In this study, we compared the global regulation of alternative polyadenylation (APA) in platinum-resistant and platinum-sensitive tissues of OC patients by analyzing a set of single-cell RNA sequencing (scRNA-seq) data from public databases and found that platinum-resistant patients exhibited global 3' untranslated region (UTR) shortening due to the different usage of polyadenylation sites (PASs). The APA regulator CSTF3 was the most significantly upregulated gene in epithelial cells of platinum-resistant OC. CSTF3 knockdown increased the sensitivity of OC cells to platinum. The lncRNA NEAT1 has two isoforms, short (NEAT1_1) and long (NEAT1_2) transcript, because of the APA processing in 3'UTR. We found that CSTF3 knockdown reduced the usage of NEAT1 proximal PAS to lengthen the transcript and facilitate the expression of NEAT1_2. Downregulation of the expression of NEAT1 (NEAT1_1/_2), but not only NEAT1_2, also increased the sensitivity of OC cells to platinum. Overexpressed NEAT1_1 reversed the platinum resistance of OC cells after knocking down CSTF3 expression. Furthermore, downregulated expression of CSTF3 and NEAT1_1, rather than NEAT1_2, was positively correlated with inactivation of the PI3K/AKT/mTOR pathway in OC cells. Together, our findings revealed a novel mechanism of APA regulation in platinum-resistant OC. CSTF3 directly bound downstream of the NEAT1 proximal PAS to generate the short isoform NEAT1_1 and was conducive to platinum resistance, which provides a potential biomarker and therapeutic strategy for platinum-resistant OC patients.

基金机构：National Natural Science Foundation of China (National Science Foundation of China)

基金资助正文：No Statement Available