Multiple omics integrative analysis identifies <i>GARS1</i> as a novel prognostic and immunological biomarker: from pan-cancer to bladder cancer

作者全名："Liu, Weihui; Wei, Chengcheng; He, Qingliu; Chen, Zhaohui; Zhuang, Wei; Guo, Yihong; Xue, Xueyi"

作者地址："[Liu, Weihui; He, Qingliu; Zhuang, Wei; Guo, Yihong] Fujian Med Univ, Affiliated Hosp 2, Dept Urol, Quanzhou 362000, Peoples R China; [Wei, Chengcheng; Chen, Zhaohui] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Urol, Wuhan 430074, Peoples R China; [Wei, Chengcheng] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 404100, Peoples R China; [Xue, Xueyi] Fujian Med Univ, Affiliated Hosp 1, Urol Res Inst, Dept Urol, Fuzhou 350005, Peoples R China"

通信作者："Guo, YH (通讯作者)，Fujian Med Univ, Affiliated Hosp 2, Dept Urol, Quanzhou 362000, Peoples R China.; Xue, XY (通讯作者)，Fujian Med Univ, Affiliated Hosp 1, Urol Res Inst, Dept Urol, Fuzhou 350005, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001294085700044

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：14

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：GARS1; Pan-cancer; Bladder cancer; Multiple omics; Immune infiltration

摘要："Glycyl-tRNA synthetase (GARS1) is differentially expressed across cancers. In this study, the value of GARS1 in the diagnosis and prognosis of various cancers was comprehensively evaluated by multiple omics integrative pan-cancer analysis and experimental verification. Through Kaplan-Meier, ROC and multiple databases, we explored GARS1 expression and prognostic and diagnostic patterns across cancers. The GARS1 relative reaction network was identified in PPI, GO, KEGG, methylation models and the genetic mutation atlas. Further research on the GARS1 value in bladder urothelial carcinoma (BLCA) was conducted by regression and nomogram models. We further analyzed the correlation between GARS1 and immune markers and cells in BLCA. Finally, in vitro experiments were used to validate GARS1 the oncogenic function of GARS1 in BLCA. We found that GARS1 was highly expressed across cancers, especially in BLCA. GARS1 expression was correlated with poor survival and had high diagnostic value in most tumor types. GARS1 is significantly associated with tRNA-related pathways whose mutation sites are mainly located on tRNA synthetase. In addition, Upregulation of GARS1 was connected with immune cell infiltration and five key MMR genes. M2 macrophages, TAMs, Th1 and T-cell exhaustion, and marker sets associated with GARS1 expression indicated specific immune infiltration in BLCA. Finally, in vitro experiments validated that GARS1 expression promotes BLCA cell proliferation and metastasis and inhibits apoptosis. Overall, GARS1 can be a novel prognostic and immunological biomarker through multiple omics integrative pan-cancer analysis. The expression of GARS1 in BLCA was positively correlated with specific immune infiltration, indicating that GARS1 might be related to the tumor immune microenvironment."

基金机构：Natural Science Foundation of Fujian Province; Quanzhou City Science and Technology Program of China [2023C008YR]; [2021J01276]; [2021J01266]; [2022J01273]

基金资助正文："This research was funded by Natural Science Foundation of Fujian Province (No. 2021J01276, No. 2021J01266, No. 2022J01273) and Quanzhou City Science and Technology Program of China (No. 2023C008YR)."