Global characterization of extrachromosomal circular DNAs in advanced high grade serous ovarian cancer

作者全名："Cen, Yixuan; Fang, Yifeng; Ren, Yan; Hong, Shiyuan; Lu, Weiguo; Xu, Junfen"

作者地址："[Cen, Yixuan; Ren, Yan; Lu, Weiguo; Xu, Junfen] Zhejiang Univ, Womens Reprod Hlth Lab Zhejiang Prov, Womens Hosp, Sch Med, Hangzhou 310006, Peoples R China; [Fang, Yifeng] Zhejiang Univ, Sch Med, Sir Run Run Shaw Hosp, Dept Gen Surg, Hangzhou 310016, Peoples R China; [Hong, Shiyuan] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [Lu, Weiguo; Xu, Junfen] Zhejiang Univ, Womens Hosp, Dept Gynecol Oncol, Sch Med, Hangzhou 310006, Peoples R China; [Lu, Weiguo] Zhejiang Univ, Ctr Canc, Hangzhou 310058, Peoples R China"

通信作者："Lu, WG; Xu, JF (通讯作者)，Zhejiang Univ, Womens Reprod Hlth Lab Zhejiang Prov, Womens Hosp, Sch Med, Hangzhou 310006, Peoples R China.; Hong, SY (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China.; Lu, WG; Xu, JF (通讯作者)，Zhejiang Univ, Womens Hosp, Dept Gynecol Oncol, Sch Med, Hangzhou 310006, Peoples R China.; Lu, WG (通讯作者)，Zhejiang Univ, Ctr Canc, Hangzhou 310058, Peoples R China."

来源：CELL DEATH & DISEASE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000783545700006

JCR分区：Q1

影响因子：9

年份：2022

卷号：13

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："High grade serous ovarian cancer (HGSOC) is the most aggressive subtype of ovarian cancer and HGSOC patients often appear with metastasis, leading to the poor prognosis. Up to date, the extrachromosomal circular DNAs (eccDNAs) have been shown to be involved in cancer genome remodeling but the roles of eccDNAs in metastatic HGSOC are still not clear. Here we explored eccDNA profiles in HGSOC by Circle-Sequencing analysis using four pairs of primary and metastatic tissues of HGSOC patients. Within the differentially expressed eccDNAs screened out by our analysis, eight candidates were validated by outward PCR and qRT-PCR analysis. Among them, DNMT1(circle10302690-10302961) was further confirmed by FISH assay and BaseScope assay, as the most significantly down-regulated eccDNA in metastatic tumors of HGSOC. Lower expression of DNMT1(circle10302690-10302961) in both primary and metastatic tumors was associated with worse prognosis of HGSOC. Taken together, our finding firstly demonstrated the eccDNAs landscape of primary and metastatic tissues of HGSOC. The eccDNA DNMT1(circle10302690-10302961) can be considered as a potential biomarker or a therapeutically clinical target of HGSOC metastasis and prognosis."

基金机构：Fundamental Research Funds for the Central Universities [2021FZZX001-43]; Beijing Kanghua Foundation for the Development of Traditional Chinese and Western Medicine [KH-2021-LLZX-016]

基金资助正文："This work was supported by the Fundamental Research Funds for the Central Universities (2021FZZX001-43) and Beijing Kanghua Foundation for the Development of Traditional Chinese and Western Medicine (KH-2021-LLZX-016). We acknowledged Beibei Wang and Dandan Song in the Center of Cryo-Electron Microscopy (CCEM), Zhejiang University for their technical assistance on TEM and SEM analysis. We acknowledged BioRender.com for providing the icons and materials in our illustrations."