Toehold-mediated nonenzymatic DNA strand displacement coupling UDG mediated PCR and multi-code magnetic beads for DNA genotyping

作者全名："Zhang, Zhang; Weng, Zhi; Yao, Juan; Liu, Dan; Zhang, Li; Zhang, Limei; Xie, Guoming"

作者地址："[Zhang, Zhang; Weng, Zhi; Liu, Dan; Zhang, Li; Xie, Guoming] Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost Educ, Chongqing 400016, Peoples R China; [Zhang, Zhang; Zhang, Limei] Southwest Med Univ, Dept Lab Med, Affiliated Hosp, Luzhou, Peoples R China; [Yao, Juan] Southwest Med Univ, Affiliated Tradit Chinese Med Hosp, Dept Lab Med, Luzhou, Peoples R China"

通信作者："Xie, GM (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost Educ, Chongqing 400016, Peoples R China."

来源：MICROCHEMICAL JOURNAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000793741700001

JCR分区：Q1

影响因子：4.8

年份：2022

卷号：178

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Multi-code magnetic beads; DNA strand displacement; UDG; G6PD genotyping; Single nucleotide variation

摘要："Small variations, even single-nucleotide variants in nucleic acid sequences may exert significant influence in many diseases. The reliable detection and quantification of DNA can provide a variety of information that has significant clinical value, including disease risk assessment, screening, diagnosis, prognosis, the selection of therapies and monitoring. Due to the fact coupling efficiency of dNTPs and purification methodology can limit the chemical synthesis of long stranded probes. We demonstrated a PCR-based method to construct a long stranded detection probe with high levels of selectivity via a strand displacement reaction with ssDNA target generates by asymmetric PCR. This detection probe was enzymatically generated from a double-stranded DNA duplex, containing a single-stranded active toehold domain. This approach was successfully implemented to genotype human glucose-6-phosphate dehydrogenase (G6PD) deficiency, focusing upon the c.1376G > T and c.1388G > A variants."

基金机构："National Natural Science Foundation of China [81672112, 81802110, 81902169]; Top Talent Project of Chongqing Medical University [BJRC 201909]"

基金资助正文："This research was supported by the National Natural Science Foundation of China (Grant No.81672112, 81802110 and 81902169) and Top Talent Project of Chongqing Medical University (BJRC 201909)."