CATCH: high specific transcriptome-focused fusion gene variants discrimination

作者全名："Yuan, Rui; Bai, Xiaopeng; Hu, Xiaolin; Zhang, Hong; Hou, Changjun; Long, Quanxin; Luo, Yang"

作者地址："[Yuan, Rui; Hou, Changjun] Chongqing Univ, Key Lab Biorheol Sci & Technol Minist Educ, State & Local Joint Engn Lab Vasc Implants, Bioengn Coll, Chongqing 400044, Peoples R China; [Yuan, Rui; Hu, Xiaolin; Zhang, Hong; Luo, Yang] Chongqing Univ, Ctr Smart Lab & Mol Med, Sch Med, Chongqing 400044, Peoples R China; [Bai, Xiaopeng; Long, Quanxin] Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing 400016, Peoples R China; [Zhang, Hong] Shandong Univ, Dept Clin Lab, Hosp 2, Jinan 250033, Shandong, Peoples R China; [Luo, Yang] Chongqing Univ, Fuling Hosp, Dept Clin Lab, Chongqing 408099, Peoples R China; [Luo, Yang] Chongqing Univ, Jiangjin Hosp, Dept Clin Lab, Chongqing 402260, Peoples R China"

通信作者："Hou, CJ (通讯作者)，Chongqing Univ, Key Lab Biorheol Sci & Technol Minist Educ, State & Local Joint Engn Lab Vasc Implants, Bioengn Coll, Chongqing 400044, Peoples R China.; Luo, Y (通讯作者)，Chongqing Univ, Ctr Smart Lab & Mol Med, Sch Med, Chongqing 400044, Peoples R China.; Long, QX (通讯作者)，Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing 400016, Peoples R China.; Luo, Y (通讯作者)，Chongqing Univ, Fuling Hosp, Dept Clin Lab, Chongqing 408099, Peoples R China.; Luo, Y (通讯作者)，Chongqing Univ, Jiangjin Hosp, Dept Clin Lab, Chongqing 402260, Peoples R China."

来源：CHEMICAL COMMUNICATIONS

ESI学科分类：CHEMISTRY

WOS号：WOS:000814231700001

JCR分区：Q2

影响因子：4.9

年份：2022

卷号：58

期号：55

开始页：7618

结束页：7621

文献类型：Article

关键词：　

摘要：We develop a CRISPR-Cas13a triggered catalytic hairpin assembly (CATCH) approach for accurate and impartial identification of variants by integrating the fusion gene-selected recognition of CRISPR-Cas13a with collateral cleavage-assisted catalytic hairpin assembly amplification. This approach achieved an accuracy of 100% in a pilot experiment involving 34 clinical samples.

基金机构："National Natural Science Foundation of China [82125022, 82072383]; Key Natural Science Foundation of Chongqing [CSTC2020JCYJ-ZDXMX0006, CSTC2020JSCX-CYLHX0001]; Key Program of Sichuan Science and Technology Department [22ZDYF0318, 2021YFQ0060]; Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) [2020FYYX018]; Fundamental Research Funds for the Central Universities [X20210295]; Chongqing Returned Overseas Students' Entrepreneurship and Innovation Support Program [CX2021010]"

基金资助正文："This work was funded by the National Natural Science Foundation of China (82125022, 82072383), the Key Natural Science Foundation of Chongqing (CSTC2020JCYJ-ZDXMX0006, CSTC2020JSCX-CYLHX0001), the Key Program of Sichuan Science and Technology Department (22ZDYF0318, 2021YFQ0060), the Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) (2020FYYX018), the Fundamental Research Funds for the Central Universities (X20210295), Chongqing Returned Overseas Students' Entrepreneurship and Innovation Support Program (CX2021010)."