Comprehensive proteomic analysis of exosome mimetic vesicles and exosomes derived from human umbilical cord mesenchymal stem cells

作者全名:"Zhang, Zhaoxia; Mi, Tao; Jin, Liming; Li, Mujie; Zhanghuang, Chenghao; Wang, Jinkui; Tan, Xiaojun; Lu, Hongxu; Shen, Lianju; Long, Chunlan; Wei, Guanghui; He, Dawei"

作者地址:"[Zhang, Zhaoxia; Mi, Tao; Jin, Liming; Li, Mujie; Zhanghuang, Chenghao; Wang, Jinkui; Tan, Xiaojun; Lu, Hongxu; Shen, Lianju; Long, Chunlan; Wei, Guanghui; He, Dawei] Chongqing Med Univ, Childrens Hosp, Dept Urol, Chongqing 400014, Peoples R China; [Zhang, Zhaoxia; Mi, Tao; Jin, Liming; Li, Mujie; Zhanghuang, Chenghao; Wang, Jinkui; Tan, Xiaojun; Lu, Hongxu; Shen, Lianju; Long, Chunlan; Wei, Guanghui; He, Dawei] Chongqing Key Lab Children Urogenital Dev & Tissu, Chongqing 400014, Peoples R China; [Zhang, Zhaoxia; Mi, Tao; Jin, Liming; Li, Mujie; Zhanghuang, Chenghao; Wang, Jinkui; Tan, Xiaojun; Lu, Hongxu; Shen, Lianju; Long, Chunlan; Wei, Guanghui; He, Dawei] China Int Sci & Technol Cooperat Base Child Dev &, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Pediat, Key Lab Child Dev & Disorders,Minist Educ, Chongqing 400014, Peoples R China"

通信作者:"He, DW (通讯作者),Chongqing Med Univ, Childrens Hosp, Dept Urol, Chongqing 400014, Peoples R China.; He, DW (通讯作者),Chongqing Key Lab Children Urogenital Dev & Tissu, Chongqing 400014, Peoples R China.; He, DW (通讯作者),China Int Sci & Technol Cooperat Base Child Dev &, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Pediat, Key Lab Child Dev & Disorders,Minist Educ, Chongqing 400014, Peoples R China."

来源:STEM CELL RESEARCH & THERAPY

ESI学科分类:MOLECULAR BIOLOGY & GENETICS

WOS号:WOS:000826088500009

JCR分区:Q1

影响因子:7.5

年份:2022

卷号:13

期号:1

开始页: 

结束页: 

文献类型:Article

关键词:Mesenchymal stem cells; Exosomes; Exosome mimetic vesicles; Proteomics

摘要:"Background Exosomes derived from mesenchymal stem cells (MSCs) have shown to have effective application prospects in the medical field, but exosome yield is very low. The production of exosome mimetic vesicles (EMVs) by continuous cell extrusion leads to more EMVs than exosomes, but whether the protein compositions of MSC-derived EMVs (MSC-EMVs) and exosomes (MSC-exosomes) are substantially different remains unknown. The purpose of this study was to conduct a comprehensive proteomic analysis of MSC-EMVs and MSC-exosomes and to simply explore the effects of exosomes and EMVs on wound healing ability. This study provides a theoretical basis for the application of EMVs and exosomes. Methods In this study, EMVs from human umbilical cord MSCs (hUC MSCs) were isolated by continuous extrusion, and exosomes were identified after hUC MSC ultracentrifugation. A proteomic analysis was performed, and 2315 proteins were identified. The effects of EMVs and exosomes on the proliferation, migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) were evaluated by cell counting kit-8, scratch wound, transwell and tubule formation assays. A mouse mode was used to evaluate the effects of EMVs and exosomes on wound healing. Results Bioinformatics analyses revealed that 1669 proteins in both hUC MSC-EMVs and hUC MSC-exosomes play roles in retrograde vesicle-mediated transport and vesicle budding from the membrane. The 382 proteins unique to exosomes participate in extracellular matrix organization and extracellular structural organization, and the 264 proteins unique to EMVs target the cell membrane. EMVs and exosomes can promote wound healing and angiogenesis in mice and promote the proliferation, migration and angiogenesis of HUVECs. Conclusions This study presents a comprehensive proteomic analysis of hUC MSC-derived exosomes and EMVs generated by different methods. The tissue repair function of EMVs and exosomes was herein verified by wound healing experiments, and these results reveal their potential applications in different fields based on analyses of their shared and unique proteins."

基金机构:Innovation Leading Talent Project of Chongqing [CQYC20200303139]

基金资助正文:This research was funded by Innovation Leading Talent Project of Chongqing (Grant Number CQYC20200303139).