Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries

作者全名："Weng, Luting; Wu, Lang; Guo, Rongjuan; Ye, Jiajia; Liang, Wen; Wu, Wei; Chen, Liang; Yang, Deqin"

作者地址："[Weng, Luting; Wu, Lang; Guo, Rongjuan; Ye, Jiajia; Liang, Wen; Chen, Liang; Yang, Deqin] Chongqing Med Univ, Stomatol Hosp, 426 Songshi North Rd, Chongqing 401147, Peoples R China; [Weng, Luting; Wu, Lang] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Guo, Rongjuan; Ye, Jiajia; Liang, Wen] Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing 401147, Peoples R China; [Wu, Wei] Chongqing Univ, Bioengn Coll, 174 Shazhengjie, Chongqing 400044, Peoples R China"

通信作者："Chen, L; Yang, DQ (通讯作者)，Chongqing Med Univ, Stomatol Hosp, 426 Songshi North Rd, Chongqing 401147, Peoples R China.; Wu, W (通讯作者)，Chongqing Univ, Bioengn Coll, 174 Shazhengjie, Chongqing 400044, Peoples R China."

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000836341800006

JCR分区：Q1

影响因子：10.2

年份：2022

卷号：20

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Streptococcus mutans; Lactobacillus; Nanoparticles; Cariogenic biofilm; Cell membrane

摘要："Background: Due to their prevalence, dental caries ranks first among all diseases endangering human health. Therefore, the prevention of caries is of great significance, as caries have become a serious public health problem worldwide. Currently, using nanoscale drug delivery systems to prevent caries has received increased attention. However, the preventive efficacy of these systems is substantially limited due to the unique physiological structure of cariogenic biofilms. Thus, novel strategies aimed at combating cariogenic biofilms to improve preventive efficiency against caries are meaningful and very necessary. Herein, inspired by cell membrane coating technology and Lactobacillus strains, we coated triclosan (TCS)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (TCS@PLGA-NPs) with an envelope of Lactobacillus (LA/TCS@PLGA-NPs) and investigated their potential as a nanoparticle delivery system against cariogenic biofilms and dental caries. Results: LA/TCS@PLGA-NPs were successfully prepared with favorable properties, including a coated envelope, controllable size, negative charge, sustained drug-release kinetics and so on. The LA/TCS@PLGA-NPs inherited native properties from the source cell surface, thus the LA/TCS@PLGA-NPs adhered to S. mutans, integrated into the S. mutans biofilm, and interfered with the biofilm formation of S. mutans. The nanoparticles significantly inhibited the activity, biomass and virulence gene expression of S. mutans biofilms in vitro. Additionally, LA/TCS@PLGA-NPs exhibited a long-lasting inhibitory effect on the progression of caries in vivo. The safety performance of the nanoparticles is also favorable. Conclusions: Our findings reveal that the antibiofilm effect of LA/TCS@PLGA-NPs relies not only on the inheritance of native properties from the Lactobacillus cell surface but also on the inhibitory effect on the activity, biomass and virulence of S. mutans biofilms. Thus, these nanoparticles could be considered feasible candidates for a new class of effective drug delivery systems for the prevention of caries. Furthermore, this work provides new insights into cell membrane coating technology and presents a novel strategy to combat bacterial biofilms and associated infections."

基金机构："National Natural Science Foundation of China [31970783, 81700958]; China Postdoctoral Science Foundation [2020M683271]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0034]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (Grant No.31970783 and Grant No.81700958), China Postdoctoral Science Foundation (Grant No.2020M683271) and Program for Youth Innovation in Future Medicine, Chongqing Medical University (W0034)."