Dancing in local space: rolling hoop orbital amplification combined with local cascade nanozyme catalytic system to achieve ultra-sensitive detection of exosomal miRNA

作者全名："Gao, Xin; Wu, Haiping; Li, Yujian; Zhang, Lu; Song, Mingxuan; Fu, Xuhuai; Chen, Rui; Ding, Shijia; Zeng, Jiawei; Li, Jia; Liu, Ping"

作者地址："[Gao, Xin; Wu, Haiping; Zhang, Lu; Song, Mingxuan; Chen, Rui; Ding, Shijia; Liu, Ping] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Li, Yujian] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Fu, Xuhuai] Chongqing Univ, Chongqing Canc Hosp, Chongqing Canc Inst, Dept Clin Lab,Canc Hosp, Chongqing 400030, Peoples R China; [Zeng, Jiawei] Univ Elect Sci & Technol China, Mianyang Cent Hosp, Sch Med, Dept Clin Lab, Mianyang 621000, Sichuan, Peoples R China; [Li, Jia] Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Liu, Ping] Biosci Tianjin Diagnost Technol CO LTD, Tianjin 300399, Peoples R China"

通信作者："Zeng, JW (通讯作者)，Univ Elect Sci & Technol China, Mianyang Cent Hosp, Sch Med, Dept Clin Lab, Mianyang 621000, Sichuan, Peoples R China.; Li, J (通讯作者)，Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Liu, P (通讯作者)，Biosci Tianjin Diagnost Technol CO LTD, Tianjin 300399, Peoples R China."

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000836341800008

JCR分区：Q1

影响因子：10.2

年份：2022

卷号：20

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Rolling hoop orbital amplification; Local cascade nanozyme catalytic system; Exo-miRNA; ECL biosensor; Ultra-sensitive detection

摘要："The exosomal miRNA (exo-miRNA) derived from tumor cells contains rich biological information that can effectively aid in the early diagnosis of disease. However, the extremely low abundance imposes stringent requirements for accurate detection techniques. In this study, a novel, protease-free DNA amplification strategy, known as""Rolling Hoop Orbital Amplification"" (RHOA), was initially developed based on the design concept of local reaction and inspired by the childhood game of rolling iron ring. Benefiting from the local space constructed by the DNA orbital, the circular DNA enzyme rolls directionally and interacts efficiently with the amplification element, making it nearly 3-fold more productive than conventional free-diffusion amplification. Similarly, the localized cascade nanozyme catalytic system formed by bridging DNA probes also exhibits outperformed than free ones. Therefore, a localized energized high-performance electrochemiluminescence (ECL) biosensor was constructed by bridging cascading nanozymes on the electrode surface through DNA probes generated by RHOA, with an impressive limit of detection (LOD) of 1.5 aM for the detection of exosomal miRNA15a-5p and a stable linearity over a wide concentration range from 10(-2) to 10(8) fM. Thus, this work is a focused attempt at the localized reaction, which is expected to provide a reliable method for accurately detecting of exo-miRNAs."

基金机构：National Natural Science Foundation of China [81873980]; Open project of the Key Laboratory of Tropical Disease Prevention and Control of the National Health Commission of Hainan Medical College [2022NHCTDCKFKT21002]; Chongqing Medical University Graduate Talent Training Program [BJRC202104]; Project Fund of Sichuan Provincial Health Commission [19ZD010]

基金资助正文："This work was funded by the National Natural Science Foundation of China (81873980), Open project of the Key Laboratory of Tropical Disease Prevention and Control of the National Health Commission of Hainan Medical College (2022NHCTDCKFKT21002), Chongqing Medical University Graduate Talent Training Program (BJRC202104) and Project Fund of Sichuan Provincial Health Commission (19ZD010)."