Low-intensity pulsed ultrasound/nanomechanical force generators enhance osteogenesis of BMSCs through microfilaments and TRPM7

作者全名："Yao, Huan; Zhang, Liang; Yan, Shujin; He, Yiman; Zhu, Hui; Li, Yasha; Wang, Dong; Yang, Ke"

作者地址："[Yao, Huan; Li, Yasha; Yang, Ke] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders,Chongqin, Childrens Hosp,China Int Sci & Technol Cooperat B, Pediat Res Inst,Minist Educ,Key Lab Child Dev & D, Chongqing 400014, Peoples R China; [Yao, Huan; Zhang, Liang; Yan, Shujin; He, Yiman; Zhu, Hui; Wang, Dong] Chongqing Med Univ, Affiliated Hosp 1, Dept Ultrasound, Chongqing 400016, Peoples R China"

通信作者："Yang, K (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders,Chongqin, Childrens Hosp,China Int Sci & Technol Cooperat B, Pediat Res Inst,Minist Educ,Key Lab Child Dev & D, Chongqing 400014, Peoples R China."

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000840323400001

JCR分区：Q1

影响因子：10.2

年份：2022

卷号：20

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Low-intensity pulsed ultrasound; Nanomechanical force generators; Mechanotransduction; Osteogenesis; Transient receptor potential melastatin 7

摘要："Background: Low-intensity pulsed ultrasound (LIPUS) has been reported to accelerate fracture healing, but the mechanism is unclear and its efficacy needs to be further optimized. Ultrasound in combination with functionalized microbubbles has been shown to induce local shear forces and controllable mechanical stress in cells, amplifying the mechanical effects of LIPUS. Nanoscale lipid bubbles (nanobubbles) have high stability and good biosafety. However, the effect of LIPUS combined with functionalized nanobubbles on osteogenesis has rarely been studied. Results: In this study, we report cyclic arginine-glycine-aspartic acid-modified nanobubbles (cRGD-NBs), with a particle size of similar to 500 nm, able to actively target bone marrow mesenchymal stem cells (BMSCs) via integrin receptors. cRGD-NBs can act as nanomechanical force generators on the cell membrane, and further enhance the BMSCs osteogenesis and bone formation promoted by LIPUS. The polymerization of actin microfilaments and the mechanosensitive transient receptor potential melastatin 7 (TRPM7) ion channel play important roles in BMSCs osteogenesis promoted by LIPUS/cRGD-NBs. Moreover, the mutual regulation of TRPM7 and actin microfilaments promote the effect of LIPUS/cRGD-NBs. The extracellular Ca2+ influx, controlled partly by TRPM7, could participated in the effect of LIPUS/cRGD-NBs on BMSCs. Conclusions: The nanomechanical force generators cRGD-NBs could promote osteogenesis of BMSCs and bone formation induced by LIPUS, through regulation TRPM7, actin cytoskeleton, and intracellular calcium oscillations. This study provides new directions for optimizing the efficacy of LIPUS for fracture healing, and a theoretical basis for the further application and development of LIPUS in clinical practice."

基金机构："National Natural Science Foundation of China [81771845, 81800786]"

基金资助正文：This work was supported by the National Natural Science Foundation of China (Grant Nos. 81771845 and 81800786).