Macrophages regulate vascular smooth muscle cell function during atherosclerosis progression through IL-1 beta/STAT3 signaling
作者全名："Xue, Yuzhou; Luo, Minghao; Hu, Xiankang; Li, Xiang; Shen, Jian; Zhu, Wenyan; Huang, Longxiang; Hu, Yu; Guo, Yongzheng; Liu, Lin; Wang, Lingbang; Luo, Suxin"
作者地址："[Xue, Yuzhou; Luo, Minghao; Hu, Xiankang; Li, Xiang; Shen, Jian; Huang, Longxiang; Hu, Yu; Guo, Yongzheng; Luo, Suxin] Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiol, Chongqing, Peoples R China; [Xue, Yuzhou] Peking Univ Third Hosp, Dept Cardiol, Beijing, Peoples R China; [Xue, Yuzhou] Peking Univ Third Hosp, Inst Vasc Med, Beijing, Peoples R China; [Zhu, Wenyan] Yidu Cloud Beijing Technol Co Ltd, Med Dept, Beijing, Peoples R China; [Zhu, Wenyan] Chongqing Med & Pharmaceut Coll, Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing, Peoples R China; [Liu, Lin] Chongqing Med Univ, Affiliated Hosp 1, Dept Dermatol, Chongqing, Peoples R China; [Wang, Lingbang] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped Surg, Chongqing, Peoples R China"
通信作者："Luo, SX (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiol, Chongqing, Peoples R China."
摘要："Vascular smooth muscle cells (VSMCs) play a central role in atherosclerosis progression, but the functional changes in VSMCs and the associated cellular crosstalk during atherosclerosis progression remain unknown. Here we show that scRNA-seq analysis of proximal adjacent (PA) and atherosclerotic core (AC) regions of human carotid artery plaques identifies functional alterations in macrophage-like VSMCs, elucidating the main state differences between PA and AC VSMCs. And, IL-1 beta mediates macrophage-macrophage-like VSMC crosstalk through regulating key transcription factors involved in macrophage-like VSMCs functional alterations during atherosclerosis progression. In vitro assays reveal VSMCs transdifferentiated into a macrophage-like phenotype and then functional alterations in response to macrophage-derived stimuli. IL-1 beta promots the adhesion, inflammation, and apoptosis of macrophage-like VSMCs in a STAT3 dependent manner. The current findings provide interesting insight into the macrophages-macrophage-like VSMC crosstalk, which would drive functional alterations in the latter cell type through IL-1 beta/STAT3 axis during atherosclerosis progression."
基金机构：National Natural Science Foundation of China ; General Project of Chongqing Natural Science Foundation [cstc2020jcyj-msxmX1091]; Intelligent Medicine Training Program of Chongqing Medical University [ZHYX202017]
基金资助正文："We thank Dr. Jianming Zeng (University of Macau), and all the members of his bioinformatics team, biotrainee, for generously sharing their server. We also thank Helix Medical Institute (HMI, shanghai), Weifeng Hong (Fudan University), Ping Zheng (Shanghai Jiaotong University), and Hongsheng Cheng (Huazhong University of Science and Technology) for providing technology support. We thank Dr. Ali Toramani (Scripps Research Institute) and his group for generating and publicly sharing the single-cell dataset utilized in this study. This work was supported by the National Natural Science Foundation of China (82070238), the General Project of Chongqing Natural Science Foundation (cstc2020jcyj-msxmX1091), and the Intelligent Medicine Training Program of Chongqing Medical University (ZHYX202017)."