One-step competitive assay for detection of thrombin via disassembly of diblock oligonucleotide functionalised nanogold aggregates

作者全名:"Huang, Yu; Li, Shengqiang; Liu, Cuiying; Chen, Ligang; Qian, Husun; Ho, Ho-Pui; Wu, Jiangling; Wu, Jiang; Pu, Xiaoyin"

作者地址:"[Huang, Yu; Chen, Ligang; Wu, Jiang; Pu, Xiaoyin] Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China; [Li, Shengqiang] Tianjin Univ Tradit Chinese Med, Teaching Hosp 1, Clin Lab, Tianjin 300000, Peoples R China; [Li, Shengqiang] Natl Clin Res Ctr Chinese Med Acupuncture & Moxibu, Tianjin 300000, Peoples R China; [Liu, Cuiying] Tianjin Xiqing Hosp, Dept Clin Lab, Tianjin 300000, Peoples R China; [Qian, Husun] Zunyi Med Univ, Affiliated Hosp, Dept Lab Med, Zunyi 563003, Peoples R China; [Ho, Ho-Pui] Chinese Univ Hong Kong, Dept Biomed Engn, Hong Kong, Peoples R China; [Wu, Jiangling] Chongqing Med Univ, Univ Town Hosp, Dept Clin Lab, Chongqing 401331, Peoples R China; [Wu, Jiangling] Chongqing Med Univ, Univ Town Hosp, Med Sci Res Ctr, Chongqing, Peoples R China; [Huang, Yu; Wu, Jiang; Pu, Xiaoyin] Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China"

通信作者:"Huang, Y (通讯作者),Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China.; Wu, JL (通讯作者),Chongqing Med Univ, Univ Town Hosp, Dept Clin Lab, Chongqing 401331, Peoples R China.; Wu, JL (通讯作者),Chongqing Med Univ, Univ Town Hosp, Med Sci Res Ctr, Chongqing, Peoples R China.; Huang, Y (通讯作者),Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China."

来源:SENSORS AND ACTUATORS B-CHEMICAL

ESI学科分类:CHEMISTRY

WOS号:WOS:000904410500009

JCR分区:Q1

影响因子:8.4

年份:2023

卷号:376

期号: 

开始页: 

结束页: 

文献类型:Article

关键词:Surface plasmon resonance; Gold nanoparticle aggregates; Competitive bioassay; Biosensing

摘要:"In this study, we developed a simple, sensitive and cost effective sensing strategy for thrombin. Two polyadenine diblock oligonucleotides, one containing a thrombin aptamer (TA) and the other one a partial complementary sequence to thrombin aptamer (TAC), were designed to anchor on gold nanoparticle (AuNP) surface. The hybridisation of these two DNA strands promoted the assembly of some AuNPs together and formed AuNP ag-gregates. The introduction of thrombin competitively bound to its aptamer and triggered the disassembly of AuNP aggregates. The change in plasmon absorption of AuNPs generated significant signal without requiring labeling or preprocessing steps. This simple one-step competitive assay had been demonstrated for the detection of thrombin with a detection limit of 0.81 pM and a dynamic range from 10 pM to 10 nM. Furthermore, this method was successfully demonstrated to perform analysis in 10-fold diluted serum solutions without sophis-ticated instruments. This sensitive and convenient strategy is expected to provide a prospective alternative in clinical application in a fast and straight forward manner."

基金机构:Project of science and technology research program of Chongqing Education Commission of China [KJQN202200431]; Scientific Research Funding; First Teaching Hospital of Tianjin University of Traditional Chinese Medicine; Talents Project of University -Town Hospital of Chongqing Medical University

基金资助正文:"This work was funded by the Project of science and technology research program of Chongqing Education Commission of China (Grant No. KJQN202200431) , the Scientific Research Funding (account num- ber: 2019JC05) , the ?new development project ? of the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine (201905) and Talents Project of University -Town Hospital of Chongqing Medical University."