Development of a 7-miRNA prognostic signature for patients with bladder cancer
作者全名："Xv, Yingjie; Qiu, Ming; Liu, Zhaojun; Xiao, Mingzhao; Wang, Fen"
作者地址："[Xv, Yingjie; Xiao, Mingzhao] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400016, Yuzhong, Peoples R China; [Qiu, Ming] Peoples Hosp Dazu, Dept Urol, Chongqing 402360, Dazu, Peoples R China; [Liu, Zhaojun] Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiol, Chongqing 400016, Yuzhong, Peoples R China; [Wang, Fen] Peoples Hosp Dazu, Dept Pathol, Chongqing 402360, Dazu, Peoples R China"
通信作者："Xiao, MZ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400016, Yuzhong, Peoples R China.; Wang, F (通讯作者)，Peoples Hosp Dazu, Dept Pathol, Chongqing 402360, Dazu, Peoples R China."
ESI学科分类：MOLECULAR BIOLOGY & GENETICS
关键词：bladder cancer; miRNAs; prognostic signature; risk score; nomogram
摘要："Background: Bladder carcinoma (BC) represents one of the most prevalent malignant cancers, while predicting its clinical outcomes using traditional indicators is difficult. This study aimed to develop a miRNA signature for the prognostic prediction of patients with BC.Materials and Methods: MiRNAs that expressed differentially were identified between 413 BC and 19 non-tumor patients, whose prognostic values were evaluated using univariate and multivariate Cox regression analyses. The independent prognostic factors were screened out and were used to establish a signature. The risk score of the signature was calculated. Receiver operating characteristic (ROC) curves and Kaplan-Meier curves were used to verify the predictive performance of the miRNA signature and the risk score. A nomogram was constructed which integrated with the miRNA signature and clinical parameters. Experiments were performed. Results: 7 prognosis related miRNAs were selected as independent risk factors, and a 7-miRNA signature was constructed, with an area under ROC (AUC) of 0.721. The 7-miRNA-signature based risk score acts as an independent prognostic factor, with satisfactory predictive performance (AUC = 0.744). Increased miR-337-3p expressions were detected in tumor samples and BC cell lines than in non-tumorigenic tissues and cell lines. Experiments suggested that miR-337-3p induces the proliferation, migration, and invasion of BC cells.Conclusion: The constructed 7-miRNA signature is a promising biomarker for predicting the prognosis of patients with BC, and miR-337-3p may act as a candidate therapeutic target in BC treatments."
基金机构：Chongqing Municipal Health Committee; [CQYC2020020318]
基金资助正文：Funding The project was funded by the Chongqing Municipal Health Committee (CQYC2020020318) .