Stress CMR T1-mapping technique for assessment of coronary microvascular dysfunction in a rabbit model of type II diabetes mellitus: Validation against histopathologic changes

作者全名："Ma, Peisong; Liu, Juan; Hu, Yurou; Chen, Lin; Liang, Hongqin; Zhou, Xiaoyue; Shang, Yongning; Wang, Jian"

作者地址："[Ma, Peisong; Hu, Yurou; Chen, Lin; Liang, Hongqin; Wang, Jian] Army Med Univ, Mil Med Univ 3, Southwest Hosp, Dept Radiol, Chongqing, Peoples R China; [Ma, Peisong] Chongqing Med Univ, Affiliated Hosp 1, Dept Radiol, Chongqing, Peoples R China; [Liu, Juan; Shang, Yongning] Army Med Univ, Mil Med Univ 3, Southwest Hosp, Dept Ultrasound, Chongqing, Peoples R China; [Zhou, Xiaoyue] Siemens Healthineers Ltd, MR Collaborat, Shanghai, Peoples R China"

通信作者："Wang, J (通讯作者)，Army Med Univ, Mil Med Univ 3, Southwest Hosp, Dept Radiol, Chongqing, Peoples R China.; Shang, YN (通讯作者)，Army Med Univ, Mil Med Univ 3, Southwest Hosp, Dept Ultrasound, Chongqing, Peoples R China."

来源：FRONTIERS IN CARDIOVASCULAR MEDICINE

ESI学科分类：　

WOS号：WOS:000922608500001

JCR分区：Q2

影响因子：3.6

年份：2023

卷号：9

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：adenosine triphosphate; cardiovascular magnetic resonance; coronary microvascular dysfunction; microvascular density; T1-mapping; type 2 diabetes mellitus

摘要："BackgroundCoronary microvascular dysfunction (CMD) is an early character of type 2 diabetes mellitus (T2DM), and is indicative of adverse events. The present study aimed to validate the performance of the stress T1 mapping technique on cardiac magnetic resonance (CMR) for identifying CMD from a histopathologic perspective and to establish the time course of CMD-related parameters in a rabbit model of T2DM. MethodsNew Zealand white rabbits (n = 30) were randomly divided into a control (n = 8), T2DM 5-week (n = 6), T2DM 10-week (n = 9), and T2DM 15-week (n = 7) groups. The CMR protocol included rest and adenosine triphosphate (ATP) stress T1-mapping imaging using the 5b(20b)3b-modified look-locker inversion-recovery (MOLLI) schema to quantify stress T1 response (stress Delta T1), and first-pass perfusion CMR to quantify myocardial perfusion reserve index (MPRI). After the CMR imaging, myocardial tissue was subjected to hematoxylin-eosin staining to evaluate pathological changes, Masson trichrome staining to measure collagen volume fraction (CVF), and CD31 staining to measure microvascular density (MVD). The associations between CMR parameters and pathological findings were determined using Pearson correlation analysis. ResultsThe stress Delta T1 values were 6.21 +/- 0.59%, 4.88 +/- 0.49%, 3.80 +/- 0.40%, and 3.06 +/- 0.54% in the control, T2DM 5-week, 10-week, and 15-week groups, respectively (p < 0.001) and were progressively weakened with longer duration of T2DM. Furthermore, a significant correlation was demonstrated between the stress Delta T1 vs. CVF and MVD (r = -0.562 and 0.886, respectively; p < 0.001). ConclusionThe stress T1 response correlated well with the histopathologic measures in T2DM rabbits, indicating that it may serve as a sensitive CMD-related indicator in early T2DM."

基金机构：National Natural Science Foundation of China [81971587]; Innovation Talent Promotion Program of Chongqing [4139Z2399]; Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0399]; Chongqing Postdoctoral Science Foundation [CSTB2022BSXM-JCX0032]; Chongqing Science and Health Joint Project [2021MSXM341]

基金资助正文："Funding This work was funded by the National Natural Science Foundation of China (Grant No. 81971587), the Innovation Talent Promotion Program of Chongqing (Grant No. 4139Z2399), the Natural Science Foundation of Chongqing (Grant No. cstc2020jcyj-msxmX0399), Chongqing Postdoctoral Science Foundation (Grant No. CSTB2022BSXM-JCX0032), and Chongqing Science and Health Joint Project (Grant No. 2021MSXM341)."