Exposure to Benzo(a)pyrene promotes proliferation and inhibits differentiation of stromal cells in mice during decidualization

作者全名："Xu, Hanting; Yi, Ting; Liu, Min; Gao, Rufei; Liu, Xueqing; He, Junlin; Ding, Yubin; Geng, Yanqing; Mu, Xinyi; Wang, Yingxiong; Chen, Xuemei"

作者地址："[Yi, Ting; Liu, Min; Gao, Rufei; Liu, Xueqing; He, Junlin; Ding, Yubin; Chen, Xuemei] Chongqing Med Univ, Sch Publ Hlth, Lab Reprod Biol, Chongqing 400016, Peoples R China; [Xu, Hanting; Gao, Rufei; Liu, Xueqing; He, Junlin; Ding, Yubin; Geng, Yanqing; Mu, Xinyi; Wang, Yingxiong; Chen, Xuemei] Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China; [Xu, Hanting; Geng, Yanqing; Mu, Xinyi; Wang, Yingxiong] Chongqing Med Univ, Coll Basic Med, Chongqing 400016, Peoples R China; [Chen, Xuemei] Chongqing Med Univ, Women & Childrens Hosp, Dept Obstet & Gynecol, Chongqing 401147, Peoples R China; [Yi, Ting] Chongqing Tongnan Ctr Dis Control & Prevent, Chongqing 402660, Peoples R China; [Liu, Min] Chongqing Three Gorges Med Coll, Sch Publ Hlth & Management, Chongqing 404120, Peoples R China"

通信作者："Chen, XM (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Lab Reprod Biol, Chongqing 400016, Peoples R China."

来源：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ESI学科分类：ENVIRONMENT/ECOLOGY

WOS号：WOS:000926651300001

JCR分区：Q1

影响因子：6.8

年份：2023

卷号：251

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Benzo(a)pyrene; Cell cycle; Decidualization; Proliferation; Differentiation; Polyploid

摘要："The environmental pollutant Benzo(a)pyrene (BaP) has an adverse effect on the reproductive performance of mammals. We previously showed that BaP treatment during early pregnancy damages endometrial morphology and impairs embryo implantation. Endometrial decidualization at the implantation site (IS) after embryo im-plantation is crucial for pregnancy maintenance and placental development. The balance between proliferation and differentiation in endometrial stromal cells (ESCs) is a crucial event of decidualization, which is regulated by the cell cycle. Here, we report that abnormal decidualization caused by BaP is associated with cell cycle disturbance of stromal cells. The mice in the treatment group were gavaged with 0.2 mg/kg/day BaP from day 1-8 of pregnancy, while those in control were gavaged with corn oil in parallel. BaP damaged the decidualization of ESCs and reduced the number of polyploid cells. Meanwhile, BaP up-regulated the expression of Ki67 and PCNA, affecting the differentiation of stromal cells. The cell cycle progression analysis during decidualization in vivo and in vitro showed that BaP induced polyploid cells deficiency with enhanced expressions of CyclinA(E)/ CDK2, CyclinD/CDK4 and CyclinB/CDK1, which promote the transformation of cells from G1 to S phase and simultaneously activate the G2/M phase. The above results indicated that BaP exposure accelerates cell cycle progression, promotes ESC proliferation, inhibits differentiation, and impedes proper decidualization and polyploidy development. Thus, the imbalance of ESC proliferation and differentiation would be an important mechanism for BaP-induced defective decidualization."

基金机构："National Natural Science Founda- tion of China [81573175]; Basic and Frontier Research Program of Chongqing [cstc2018jcyjAX0315]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0092]; Natural Science General Project of Chongqing Three Gorges Medical College [2019XZYB06]"

基金资助正文："This paper was supported by the National Natural Science Founda- tion of China (Grant number 81573175) , Basic and Frontier Research Program of Chongqing (Grant number cstc2018jcyjAX0315) , Program for Youth Innovation in Future Medicine, Chongqing Medical University (Grant number W0092) , and Natural Science General Project of Chongqing Three Gorges Medical College (Grant number 2019XZYB06) ."