Double-wing switch nanodevice-mediated primer exchange reaction for the activity analysis of cancer biomarker FEN1

作者全名："Chen, Siyi; Xie, Zuowei; Zhang, Wenxiu; Zhao, Shuhui; Zhao, Zixin; Wang, Xingyu; Huang, Yuqi; Yi, Gang"

作者地址："[Chen, Siyi; Xie, Zuowei; Zhang, Wenxiu; Zhao, Shuhui; Zhao, Zixin; Yi, Gang] Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Wang, Xingyu] Wuxi Peoples Hosp, Clin Lab, Chongqing 405800, Peoples R China; [Huang, Yuqi] Jiulongpo Peoples Hosp, Clin Lab, Chongqing 400050, Peoples R China"

通信作者："Yi, G (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000934827400004

JCR分区：Q1

影响因子：6.2

年份：2023

卷号：1238

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Flap endonuclease 1; Nucleic acid nanodevice; Primer exchange reaction; Tumor markers

摘要："DNA damage repair is one of the foremost factors leading to changes in tumor drug resistance. The analysis of Flap endonuclease 1 (FEN1), a kind of pivotal enzyme in various DNA metabolic pathways, has been of great support to tumor research and the development of chemotherapeutics. Nevertheless, few analytical techniques can achieve quantitative and simplified FEN1 measurement. Here, we constructed a double-wing switch nanodevice (DWSN)-mediated primer exchange technique for rapid and label-free quantification of FEN1 activity. Target FEN1 triggered the generation of numerous telomeric repeat fragments in different lengths through recognizing the three-base mismatched sites on the DWSN to release the 5 '-Flaps. Further binding to the fluorescent dye ThT resulted in significantly enhanced fluorescence. This study broke the limitation of traditional single-site identification and demonstrated good sensitivity and specificity with detection limits up to 0.55 mU. Besides, the extraordinary analytical performance allowed the method to be utilized to monitor FEN1 extracted from cells and clinical serum samples and to compare the effect of targeted FEN1 inhibitors."

基金机构：Key Project of Science and Tech-nology Research Program of Chongqing Education Commission [KJZD-K202000404]; Special Fund Project Key Laboratory of Clinical Laboratory Diagnosis (Ministry of Education)

基金资助正文：Acknowledgements This work was supported by the Key Project of Science and Tech-nology Research Program of Chongqing Education Commission (Grant No. KJZD-K202000404) and the Special Fund Project Key Laboratory of Clinical Laboratory Diagnosis (Ministry of Education) .