comCDE (Competence) Operon Is Regulated by CcpA in Streptococcus pneumoniae D39

作者全名:"Zhang, Yapeng; Zhang, Jinghui; Xiao, Jiangming; Wang, Hanyi; Yang, Rui; Guo, Xinlin; Zheng, Yuqiang; Yin, Yibing; Zhang, Xuemei"

作者地址:"[Zhang, Yapeng; Xiao, Jiangming; Wang, Hanyi; Yang, Rui; Yin, Yibing; Zhang, Xuemei] Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med Designated, Minist Educ, Chongqing, Peoples R China; [Zhang, Jinghui] Zybio Inc, Chongqing, Peoples R China; [Guo, Xinlin; Zheng, Yuqiang] Chongqing Med Univ, Dept Med Lab, Childrens Hosp, Chongqing, Peoples R China"

通信作者:"Zhang, XM (通讯作者),Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med Designated, Minist Educ, Chongqing, Peoples R China."











文献类型:Article; Early Access

关键词:Streptococcus pneumoniae D39; competence; transformation; CcpA

摘要:"Natural transformation plays an important role in the formation of drug-resistant bacteria. Exploring the regulatory mechanism of natural transformation can aid the discovery of new antibacterial targets and reduce the emergence of drug-resistant bacteria. Competence is a prerequisite of natural transformation in Streptococcus pneumoniae, in which comCDE operon is the core regulator of competence. To date, only ComE has been shown to directly regulate comCDE transcription. In this study, a transcriptional regulator, the catabolite control protein A (CcpA), was identified that directly regulated comCDE transcription. We confirmed that CcpA binds to the cis-acting catabolite response elements (cre) in the comCDE promoter region to regulate comCDE transcription and transformation. Moreover, CcpA can coregulate comCDE transcription with phosphorylated and dephosphorylated ComE. Regulation of comCDE transcription and transformation by CcpA was also affected by carbon source signals. Together, these insights demonstrate the versatility of CcpA and provide a theoretical basis for reducing the emergence of drug-resistant bacteria.IMPORTANCE Streptococcus pneumoniae is a major cause of bacterial infections in humans, such as pneumonia, bacteremia, meningitis, otitis media, and sinusitis. Like most streptococci, S. pneumoniae is naturally competent and employs this ability to augment its adaptive evolution. The current study illustrates CcpA, a carbon catabolite regulator, can participate in the competence process by regulating comCDE transcription, and this process is regulated by different carbon source signals. These hidden abilities are likely critical for adaptation and colonization in the environment."

基金机构:"National Natural Science Foundation of China [81772153, 81871698]"

基金资助正文:ACKNOWLEDGMENTS We thank Jingren Zhang for providing synthetic Janus cassette and plasmid pPEPY-PF6-lacI. This study was supported by grants 81772153 and 81871698 from the National Natural Science Foundation of China. We declare no conflict of interest.