Exopolysaccharides metabolism and cariogenesis of Streptococcus mutans biofilm regulated by antisense vicK RNA

作者全名:"Sun, Yuting; Chen, Hong; Xu, Mengmeng; He, Liwen; Mao, Hongchen; Yang, Shiyao; Qiao, Xin; Yang, Deqin"

作者地址:"[Sun, Yuting; Chen, Hong; Xu, Mengmeng; He, Liwen; Mao, Hongchen; Yang, Shiyao; Qiao, Xin; Yang, Deqin] Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing, Peoples R China; [Sun, Yuting; Chen, Hong; Xu, Mengmeng; He, Liwen; Mao, Hongchen; Yang, Shiyao; Qiao, Xin; Yang, Deqin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Sun, Yuting; Chen, Hong; Xu, Mengmeng; He, Liwen; Mao, Hongchen; Yang, Shiyao; Qiao, Xin; Yang, Deqin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Yang, Deqin] Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 4404100, Peoples R China"

通信作者:"Yang, DQ (通讯作者),Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 4404100, Peoples R China."

来源:JOURNAL OF ORAL MICROBIOLOGY

ESI学科分类:MICROBIOLOGY

WOS号:WOS:000978222800001

JCR分区:Q1

影响因子:4.5

年份:2023

卷号:15

期号:1

开始页: 

结束页: 

文献类型:Article

关键词:Streptococcus mutans; Antisense RNA; vicK gene; biofilm growth; exopolysaccharides; dental caries; bacterial genetics

摘要:"Background Streptococcus mutans (S. mutans) is a pivotal cariogenic pathogen contributing to its multiple virulence factors, one of which is synthesizing exopolysaccharides (EPS). VicK, a sensor histidine kinase, plays a major role in regulating genes associated with EPS synthesis and adhesion. Here we first identified an antisense vicK RNA (ASvicK) bound with vicK into double-stranded RNA (dsRNA). Objective This study aims to investigate the effect and mechanism of ASvicK in the EPS metabolism and cariogenesis of S. mutans. Methods The phenotypes of biofilm were detected by scanning electron microscopy (SEM), gas chromatography-mass spectrometery (GC-MS) , gel permeation chromatography (GPC) , transcriptome analysis and Western blot. Co-immunoprecipitation (Co-ip) assay and enzyme activity experiment were adopted to investigate the mechanism of ASvicK regulation. Caries animal models were developed to study the relationship between ASvicK and cariogenicity of S. mutans. Results Overexpression of ASvicK can inhibit the growth of biofilm, reduce the production of EPS and alter genes and protein related to EPS metabolism. ASvicK can adsorb RNase III to regulate vicK and affect the cariogenicity of S. mutans. Conclusions ASvicK regulates vicK at the transcriptional and post-transcriptional levels, effectively inhibits EPS synthesis and biofilm formation and reduces its cariogenicity in vivo."

基金机构:"National Natural Science Foundation of China [31970783, 32270888]; National Science Fund for Distinguished Young Scholars [82100991]; Top Talent Distinguished Professor from Chongqing Medical University [(2021) 215]; Program for Youth Innovation in Future Medicine from Chongqing Medical University [W0060]; Natural Science Foundation Project of Chongqing Science and Technology Commission [cstc2021jcyj-bshX0211]; Basic Research and Frontiers Exploration Project of Science and Technology Committee of Yuzhong District [20210124]"

基金资助正文:This research was funded by the National Natural Science Foundation of China under Grant Agreement (No. 31970783 and No. 32270888). This work was supported by a grant from National Science Fund for Distinguished Young Scholars (No. 82100991). This research was funded by the Top Talent Distinguished Professor from Chongqing Medical University [No. (2021) 215] and Program for Youth Innovation in Future Medicine from Chongqing Medical University (No. W0060). This research was funded by the Natural Science Foundation Project of Chongqing Science and Technology Commission (cstc2021jcyj-bshX0211) and the Basic Research and Frontiers Exploration Project of Science and Technology Committee of Yuzhong District (No. 20210124).