Strong red fluorescent probe for detecting chymotrypsin activity in vivo and in vitro

作者全名："Qu, Yi; Xu, Zhongsheng; Wang, Jiemen; Liu, Wei; Iqbal, Anam; Iqbal, Kanwal; Su, Yaling; Cao, Yuping; Yang, Jilu; Qin, Wenwu; Liu, Yun"

作者地址："[Qu, Yi; Wang, Jiemen; Su, Yaling; Cao, Yuping; Yang, Jilu; Qin, Wenwu] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat G, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China; [Qu, Yi; Wang, Jiemen; Su, Yaling; Cao, Yuping; Yang, Jilu; Qin, Wenwu] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Special Funct Mat & Struct Design MOE, Lanzhou, Peoples R China; [Xu, Zhongsheng; Liu, Yun] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Liu, Wei] Sichuan Univ Sci & Engn, Sch Chem & Environm Engn, Zigong 643000, Peoples R China; [Iqbal, Anam] Univ Baluchistan, Dept Chem, Quetta 87300, Pakistan; [Iqbal, Kanwal] Sardar Bahadur Khan Womens Univ, Dept Chem, Quetta 87300, Pakistan; [Qin, Wenwu] Peoples Govt Qinghai Prov, Acad Plateau Sci & Sustainabil, Xining 810016, Peoples R China; [Qin, Wenwu] Beijing Normal Univ, Xining 810016, Peoples R China"

通信作者："Qin, WW (通讯作者)，Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat G, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.; Qin, WW (通讯作者)，Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Special Funct Mat & Struct Design MOE, Lanzhou, Peoples R China.; Liu, Y (通讯作者)，Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：SENSORS AND ACTUATORS B-CHEMICAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000979453100001

JCR分区：Q1

影响因子：8.4

年份：2023

卷号：382

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Fluorescence detection; Chymotrypsin; In vivo fluorescence imaging

摘要："Chymotrypsin is a medication that is useful in treating inflammation, edema, expectoration, and corruption. It has a wide range of medical applications. Therefore, it is critical to create a chymotrypsin real-time drug tracking detection approach. The ester bond in the molecule can be severed by chymotrypsin. Intramolecular charge transfer (ICT) recovers when the ester bond in the probe breaks, resulting in fluorescence. As a result, in this research, we built and investigated the ""turn-on"" fluorescent probe NBD-1, NBD-2, and NBD-3, which are used to detect endogenous chymotrypsin, and explored the effect of molecular chain length on probe sensitivity through density functional theory (DFT) calculation and spectral testing, and screened the best probe NBD-3, which is a rare bright red fluorescence probe. The presence of endogenous chymotrypsin was subsequently discovered for the first time in the liver of mice with NBD-3 probe in confocal imaging of HeLa cells, in vivo imaging of mice, and in vitro imaging of organs in the experiment. This result confirmed that such probes could precisely detect the distribution of chymotrypsin in various organs of the organism. NBD-3's strong performance makes it possible to detect chymotrypsin medications in real time with a robust tool."

基金机构："National Natural Science Foundation of China [21771092, 81903561]; Fundamental Research Funds for the Central Univer- sities [lzujbky-2020-kb13]; Special Fund Project of Guiding Scien- tific, technological Innovation Development of Gansu Province [2019ZX-04]; 111 Project [B20027]; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China"

基金资助正文："Support The authors would like to thank the National Natural Science Foundation of China (no. 21771092 and 81903561) . This work was supported by the Fundamental Research Funds for the Central Univer- sities (lzujbky-2020-kb13) , the Special Fund Project of Guiding Scien- tific, technological Innovation Development of Gansu Province (2019ZX-04) and the 111 Project, B20027. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China, funded this work."