Multi-omics analysis reveals the evolutionary origin of diterpenoid alkaloid biosynthesis pathways in <i>Aconitum</i>

作者全名："Zhao, Dake; Zhang, Ya; Ren, Huanxing; Shi, Yana; Dong, Ding; Li, Zonghang; Cui, Guanghong; Shen, Yong; Mou, Zongmin; Kennelly, Edward J.; Huang, Luqi; Ruan, Jue; Chen, Suiyun; Yu, Diqiu; Cun, Yupeng"

作者地址："[Zhao, Dake; Dong, Ding; Li, Zonghang; Mou, Zongmin; Chen, Suiyun] Yunnan Univ, Sch Ecol & Environm Sci, Kunming 650500, Peoples R China; [Zhang, Ya; Ruan, Jue] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518000, Peoples R China; [Ren, Huanxing; Cun, Yupeng] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Translat Med Res Cognit Dev & Le, Chongqing 400014, Peoples R China; [Ren, Huanxing; Cun, Yupeng] Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China; [Shi, Yana; Huang, Luqi] Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Peoples R China; [Cui, Guanghong; Huang, Luqi] Chinese Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China; [Shen, Yong] Yunnan Agr Univ, Coll Agr & Biotechnol, Kunming 650201, Peoples R China; [Kennelly, Edward J.] CUNY, Lehman Coll, Dept Biol Sci, Bronx, NY 10468 USA; [Kennelly, Edward J.] CUNY, Grad Ctr, Bronx, NY 10468 USA; [Yu, Diqiu] Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming 650500, Peoples R China"

通信作者："Chen, SY (通讯作者)，Yunnan Univ, Sch Ecol & Environm Sci, Kunming 650500, Peoples R China.; Cun, YP (通讯作者)，Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Translat Med Res Cognit Dev & Le, Chongqing 400014, Peoples R China.; Cun, YP (通讯作者)，Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China.; Yu, DQ (通讯作者)，Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming 650500, Peoples R China."

来源：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001137576800010

JCR分区：Q1

影响因子：9.3

年份：2023

卷号：65

期号：10

开始页：2320

结束页：2335

文献类型：Article

关键词：Aconitum vilmorinianum; acute toxicity; biosynthetic pathway; diterpenoid alkaloid; evolutionary origin; genome assembly

摘要："Diterpenoid alkaloids (DAs) have been often utilized in clinical practice due to their analgesic and anti-inflammatory properties. Natural DAs are prevalent in the family Ranunculaceae, notably in the Aconitum genus. Nevertheless, the evolutionary origin of the biosynthesis pathway responsible for DA production remains unknown. In this study, we successfully assembled a high-quality, pseudochromosome-level genome of the DA-rich species Aconitum vilmorinianum (A. vilmorinianum) (5.76 Gb). An A. vilmorinianum-specific whole-genome duplication event was discovered using comparative genomic analysis, which may aid in the evolution of the DA biosynthesis pathway. We identified several genes involved in DA biosynthesis via integrated genomic, transcriptomic, and metabolomic analyses. These genes included enzymes encoding target ent-kaurene oxidases and aminotransferases, which facilitated the activation of diterpenes and insertion of nitrogen atoms into diterpene skeletons, thereby mediating the transformation of diterpenes into DAs. The divergence periods of these genes in A. vilmorinianum were further assessed, and it was shown that two major types of genes were involved in the establishment of the DA biosynthesis pathway. Our integrated analysis offers fresh insights into the evolutionary origin of DAs in A. vilmorinianum as well as suggestions for engineering the biosynthetic pathways to obtain desired DAs."

基金机构："We would like to thank the Yuxi Rongjian Information Technology Company for its cloud computing support. This research was supported by funding from the CAS ""Pioneer Hundred Talents"" Program and Strategic Priority Research Program of the Chinese Academy of [XDB31000000]; CAS ""Pioneer Hundred Talents"" Program and Strategic Priority Research Program of the Chinese Academy of Sciences [31960082, 32270395, 31960096]; National Natural Science Foundation of China [202101AS070021]; Yunnan Fundamental Research Projects"

基金资助正文："We would like to thank the Yuxi Rongjian Information Technology Company for its cloud computing support. This research was supported by funding from the CAS ""Pioneer Hundred Talents"" Program and Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000) to Y.C.; National Natural Science Foundation of China (Nos. 31960082, 32270395, and 31960096), and Yunnan Fundamental Research Projects (No. 202101AS070021) to D.Z. All authors read and approve this manuscript."