关键词： 离子通道   GABA-A受体   神经元   膜片钳  

摘要： 目的研究体外培养大鼠海马神经元在发育过程中,GABA-A受体离子通道的电生理学特性的变化规律。方法用膜片钳记录仪在体外培养的不同时期,记录大鼠海马神经元GABA-A受体离子通道全细胞通道电流、电导的变化。结果海马神经元GABA-A受体全细胞离子通道平均电流,在培养的第3天、第7天、第10天分别为12.04±3.12、18.21±3.68和20.15±4.03 pA,相对于第3天,第7天和第10天分别增加了50%和70%。平均电流从10%到90%的上升时间为2.93±0.23 ms,电流衰减呈现单指数衰减,衰减的时间常数为16.39±4.23ms。离子通道电导,在膜电位钳制于氯反转电位以下时为20.60±2.46 pS,反转电位以上时为50.83±8.51 pS。结论体外培养海马神经元的GABA-A受体全细胞离子通道平均电流随培养时间延长逐渐增加,到培养10时达最大,同时离子通道电导在不同钳制电位下显著不同。

关键词： 多发性神经病   疑似病例   电生理学   临床应用   腱反射   脊髓病   诊断评估   感觉异常   肌无力   神经传导  

摘要： Although paresthesias of the distal lower limbs are characteristic features of polyneuropathy, they may also herald the presence of a focal neuropathy, polyra diculopathy, or myelopathy. Electromyography and nerve conduction studies (EMG/N CS) are widely used in the evaluation of such symptoms, but their utility has no t been subjected to vigorous scrutiny. We investigated the clinical impact of th e electrodiagnostic consultation in assessing suspected polyneuropathy. When com pared with the clinical impression, the result of the electrodiagnostic consulta tion was confirmatory in only 39%of all patients, and changed the diagnosis or uncovered an additional diagnosis in 43%. An alternative diagnosis was likely w hen either weakness was present (75%) or the Achilles stretch reflex was preser ved (48%). These data support the use of EMG/NCS in the diagnostic evaluation o f patients presenting with distal paresthesias, especially in those with preserv ed Achilles reflexes or motor deficits.

关键词： 打击乐   乐器   手臂   研究内容   思维方式   神经生理学   心理学   作品   教学   音乐学院  

摘要： 思维是哲学、心理学、神经生理学及其他学科的重要研究内容,本文不探讨“思维是什么?”,而是表述具体思维方式在打击乐的学习及教学中的应用, 这首先基于我在教学中的感触。一、胸有成竹在教学时,首先,我们会告诉学生站立或就坐的位置、身体与乐器的距离,槌与乐器的角度、手与槌接触的位置、手臂与槌的方位。

关键词： 牙髓   树脂充填   疼痛反应   电生理学研究   酸蚀   痛觉反应   神经痛   放电频率   刺激   变化  

摘要： 目的　研究树脂直接充填深窝洞引起牙髓神经的即刻疼痛反应。方法　在20只猫下尖牙上进行深 窝洞的树脂直接充填,测量猫下齿槽神经痛觉传入神经C纤维在树脂充填过程中的诱发放电变化。结果　在酸 蚀、涂布粘接剂、光固化粘接剂和光固化充填树脂过程中观察到,C纤维产生明显的放电变化,与刺激前静息状态 相比,放电频率由0.80±0.35Hz分别增加到30.52±13.43、34.83±16.15、54.50±20.01和36.69±15.04Hz,经统 计学分析,均有显著性差异(P<0.05)。结论　确定了树脂充填过程中在酸蚀、涂布粘接剂、光固化粘接剂和光固 化充填树脂的各环节能刺激牙髓神经产生疼痛反应,提示临床在树脂直接充填深窝洞时,应在术中采取措施减少 疼痛。

关键词： 飞行员   晕厥   心理生理学  

摘要： 某歼教-5飞行员，25岁，飞行时间720h。在一次昼间简单特技飞行中，坐前舱操纵飞机，在4000m做60°盘旋时出现头昏、眼花、心悸、出汗、乏力等症状。约2min后意识丧失，持续约15s。当时天气状况良好，飞机加速度值2～3G。出现上述不适症状时该飞行员及时提醒后舱飞行员接替驾驶。后者立即报告地面指挥员，提前返场，安全着陆。

关键词： 脱髓鞘性   环孢霉素   多发性神经炎   谷浓度   红细胞糖苷脂   正中神经   临床症状   神经生理学   葡糖醛酸基   总蛋白量  

摘要： To investigate the therapeutic efficacy of cyclosporin A (CyA) in the treatmen t of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP), a microem ulsion form of this drug (Neoral) was orally given to seven patients with the disease who were unresponsive or resistant to conventional therapies.The daily d ose of CyA was carefully controlled in order to keep the plasma trough concentra tion between 100 and 150 ng/ml. Within 1 month of initiation of CyA, all patient s subjectively showed improvement of clinical symptoms, while both modified Rank in and INCAT disability scores were significantly decreased (p < 0.05) and grip strength was significantly increased (p < 0.05) 3 months after initiation compar ed with before. Total protein in the cerebrospinal fluid was significantly decre ased 3 and 6 months after starting CyA (p < 0.05). Although the maximal motor ne rve conduction velocity showed a significant improvement in the median nerve 1 t o 1.5 years after commencement of CyA (p < 0.05), there were no significant chan ges in any other neurophysiological parameters. One patient with anti sulphoglu curonyl paragloboside IgM antibodies gradually became resistant to CyA, but the rest have since been in good neurological con dition without complications ascribable to this drug. These results suggest that oral CyA may be effective even for refractory cases with CIDP. CyA should be ac tively considered as a therapeutic option when patients with CIDP are resistant to conventional treatment.