Construction of Dual-Target Recognition-Based Specific MicroRNA Detection Method for Acute Pancreatitis Analysis

作者全名："Sun, Chunhong; Rong, Yuan; Yang, Zeping; She, Dong; Gong, Mingwei"

作者地址："[Sun, Chunhong; Rong, Yuan; Yang, Zeping; She, Dong; Gong, Mingwei] Chongqing Med Univ, Emergency Dept, Affiliated Hosp 3, 1 Huixing Shuanghu Branch Rd, Chongqing 401120, Peoples R China"

通信作者："Gong, MW (通讯作者)，Chongqing Med Univ, Emergency Dept, Affiliated Hosp 3, 1 Huixing Shuanghu Branch Rd, Chongqing 401120, Peoples R China."

来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000773835400001

JCR分区：Q3

影响因子：3

年份：2022

卷号：194

期号：7

开始页：3136

结束页：3144

文献类型：Article

关键词：Acute pancreatitis; Duplex-specific nuclease; DNA enzyme; Molecular beacon; DNAzyme

摘要："MicroRNAs (miRNAs) play crucial roles in regulating various biological processes and are considered promising biomarkers for clinical diagnosis and therapy of acute pancreatitis. Herein, we present a duplex-specific nuclease (DSN enzyme) and DNAzyme-assisted fluorescent miRNA detections assay that can provide improved detection specificity due to a design of dual-target recognition and a comparable sensitivity. The dual-target recognitions are composed of (i) miRNA unfold hairpin structure toehold to form DNA-RNA duplex, among which the DNA section will be digested by DSN enzyme, releasing miRNA to participant in a next recycle. (ii) After DNAzyme-based nicking site formation in loop section of molecular beacon (MB), miRNA can bind with the loop section of MB and gradually unfold MB probe, generating fluorescence signals. With this general principle, distinct discrimination capability towards even one base pair mismatch of homogenous miRNA is obtained, showing a promising prospect in clinical diagnosis and therapy of acute pancreatitis."

基金机构：Third Affiliated Hospital of Chongqing Medical University

基金资助正文：The authors thank the financial and equipment support from The Third Affiliated Hospital of Chongqing Medical University.