Green synthesis and characterization of gold nanoparticles from Pholiota adiposa and their anticancer effects on hepatic carcinoma

作者全名："Yang, Zhongwei; Liu, Zijing; Zhu, Junmo; Xu, Jie; Pu, Youwei; Bao, Yixi"

作者地址："[Yang, Zhongwei; Zhu, Junmo; Xu, Jie; Pu, Youwei; Bao, Yixi] Chongqing Med Univ, Dept Clin Lab, Affiliated Hosp 2, Chongqing, Peoples R China; [Liu, Zijing] Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 3, Chongqing, Peoples R China"

通信作者："Bao, YX (通讯作者)，Chongqing Med Univ, Dept Clin Lab, Affiliated Hosp 2, Chongqing, Peoples R China."

来源：DRUG DELIVERY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000777047600001

JCR分区：Q1

影响因子：6

年份：2022

卷号：29

期号：1

开始页：997

结束页：1006

文献类型：Article

关键词：Pholiota adiposa polysaccharides; gold nanoparticles; immunoregulation; liver cancer

摘要："Gold nanoparticles (AuNPs) were successfully fabricated by Pholiota adiposa polysaccharide (PAP-1a) without employing any other chemicals. The physical and chemical properties of PAP-AuNPs were determined using transmission electron microscopy (TEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDXR), Fourier-transform infrared spectroscopy (FT-IR), and atomic force microscopy (AFM). In an attempt to analyze the immune regulation, antitumor effect, and biological safety, the production of NO and TNF-alpha, IL-12p70, and IL-1 beta from RAW264.7 as well as the proliferation of RAW264.7 were detected in vitro. Flow cytometry was conducted to determine the ratio of the CD4(+)/CD8(+) cell in peripheral blood and immunohistochemical analysis involving hematoxylin and eosin (H&E) and proliferating cell nuclear antigen (PCNA) staining were conducted in vivo. The results of this study showed that PAP-AuNPs had a significantly improved immune regulation and anti-tumor effect in comparison to PAP-1a alone. PAP-AuNPs showed no toxicity both in vivo and in vitro. This study demonstrates a useful application of PAP-AuNPs as a novel nanomedicine for hepatic carcinoma."

基金机构："National Natural Science Foundation of China [81773940, 81473388]; Natural Science Foundation of Chongqing Province of China [cstc2021jcyj-msxmX0094]"

基金资助正文：This research was supported by the National Natural Science Foundation of China [Nos. 81773940 and 81473388] and the Natural Science Foundation of Chongqing Province of China [No. cstc2021jcyj-msxmX0094].