Self-electrochemiluminescence biosensor based on CRISPR/Cas12a and PdCuBP@luminol nanoemitter for highly sensitive detection of cytochrome c oxidase subunit III gene of acute kidney injury

作者全名:"Liu, Changjin; Ren, Lei; Li, Xinmin; Fan, Ningke; Chen, Junman; Zhang, Decai; Yang, Wei; Ding, Shijia; Xu, Wenchun; Min, Xun"

作者地址:"[Liu, Changjin; Min, Xun] Zunyi Med Univ, Affiliated Hosp, Dept Lab Med, Zunyi 563003, Peoples R China; [Liu, Changjin; Li, Xinmin; Fan, Ningke; Chen, Junman; Zhang, Decai; Yang, Wei; Ding, Shijia; Xu, Wenchun] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Ren, Lei] Chongqing Med Univ, Affiliated Hosp 1, Hlth Management Ctr, Chongqing 400016, Peoples R China"

通信作者:"Min, X (通讯作者),Zunyi Med Univ, Affiliated Hosp, Dept Lab Med, Zunyi 563003, Peoples R China.; Xu, WC (通讯作者),Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源:BIOSENSORS & BIOELECTRONICS

ESI学科分类:CHEMISTRY

WOS号:WOS:000789665800002

JCR分区:Q1

影响因子:12.6

年份:2022

卷号:207

期号: 

开始页: 

结束页: 

文献类型:Article

关键词:Cytochrome c oxidase subunit III gene; Acute kidney injury; Electrochemiluminescence biosensor; Nanoemitter; <p>CRISPR/Cas12a</p>

摘要:"ABSTR A C T The cytochrome c oxidase subunit III (COX III) gene is a powerful biomarker for the early diagnosis of acute kidney injury. However, current methods for COX III gene detection are usually laborious and time-consuming, with limited sensitivity. Herein, we report a novel self-electrochemiluminescence (ECL) biosensor for highly sensitive detection of the COX III gene based on CRISPR/Cas12a and nanoemitters of luminol-loaded multi-component metal-metalloid PdCuBP alloy mesoporous nanoclusters. The nanoemitter with excellent self-ECL in neutral media exhibited a high specific surface area for binding luminol and outstanding oxidase-like catalytic activity toward dissolved O2. Meanwhile, the CRISPR/Cas12a system, as a target-trigger, was employed to specifically recognize the COX III gene and efficiently cleave the interfacial quencher of dopamine-labeled hairpin DNA. As a result, the ECL biosensor showed superior analytical performance for COX III gene detec-tion without exogenous coreactant. Benefiting from the high-efficiency ECL emission of the nanoemitter and Cas12a-mediated interfacial cleavage of the quencher, the developed ECL biosensor exhibited high sensitivity to COX III with a low detection limit of 0.18 pM. The established ECL biosensing method possessed excellent practical performance in urine samples. Meaningfully, the proposed strategy presents promising prospects for nucleic acid detection in the field of clinical diagnostics."

基金机构:"National Natural Science Foundation of China [32060035, 81873980, 81802079]; Science and Tech-nology Project of Guizhou [[2022] 654]; Science and Technology Fund of Guizhou Health Commission [cstc2015shmszx120098]; Society and Livelihood Foundation of Chongqing [2018QNXM003]; Chongqing Health Commission [[2018] 103, (2019] 105)]; Science and Technology Program of Zunyi [BJRC202008]; Chongqing Medical University Graduate Talent Training Program"

基金资助正文:"Acknowledgements This work was funded by the National Natural Science Foundation of China (Nos. 32060035, 81873980 and 81802079) , Science and Tech-nology Project of Guizhou (No. Qiankehejichu-ZK [2022] 654) , Science and Technology Fund of Guizhou Health Commission (No. gzwkj2022-175) , Society and Livelihood Foundation of Chongqing (No. cstc2015shmszx120098) , Chongqing Health Commission (No. 2018QNXM003) , Science and Technology Program of Zunyi (No. Zun-shikeheshezi [2018] 103 and [2019] 105) , and Chongqing Medical University Graduate Talent Training Program (No. BJRC202008) ."