Blocker displacement amplification mediated PCR based screen-printed carbon electrode biosensor and lateral flow strip strategy for CYP2C19*2 genotyping

作者全名："Yao, Juan; Zhang, Zhang; Huang, Xiaoling; Guo, Yongcan"

作者地址："[Yao, Juan; Huang, Xiaoling; Guo, Yongcan] Southwest Med Univ, Dept Lab Med, Affiliated Tradit Chinese Med Hosp, Luzhou, Peoples R China; [Zhang, Zhang] Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost Educ, Chongqing, Peoples R China"

通信作者："Guo, YC (通讯作者)，Southwest Med Univ, Dept Lab Med, Affiliated Tradit Chinese Med Hosp, Luzhou, Peoples R China.; Zhang, Z (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost Educ, Chongqing, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:000789665800006

JCR分区：Q1

影响因子：12.6

年份：2022

卷号：207

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Blocker displacement reaction; Lateral flow strip assay; Screen-printed carbon electrode; Personalized medicine

摘要："Single nucleotide variants in CYP2C19*2 are associated with clopidogrel resistance in coronary heart disease. In order the guidance the dosage of drug and personalized medicine, blocker displacement amplification was first used to specific amplify G site and A site alleles. For electrochemical strategy, forward primers were labeled electrochemical active methyl blue and ferrocene, generates signals on-0.26 for G site and 0.22 V for A site. For lateral flow strip assay, primers with specific modification were used to generates unique color in test line 1 for G site and test line 2 for A site. In conclusion, we developed a sensitive screen-printed carbon electrodes based electrochemical sensor and gold nano particle based lateral flow strip assay strategy to successfully genotyping CYP2C19*2 GG, GA and AA genotype. The proposed method can realize CYP2C19*2 analysis from multiple biological samples including whole blood, buccal swab, saliva and hair root, and showed good consistency with Sequencing. Due to the fact our proposed strategy merely relies on thermal cycler instrument and visual strip detection, this platform shows great potential in source-limited regions genotyping."

基金机构："National Natural Science Foun-dation of China [81802110, 81902169]; Applied Basic Research Foundation of Sichuan Provincial Science and Technology Department [2021JY0240]"

基金资助正文：Acknowledgements This research was supported by the National Natural Science Foun-dation of China (No.81802110 and 81902169) and Applied Basic Research Foundation of Sichuan Provincial Science and Technology Department (No. 2021JY0240) .