New anti-tumor strategy based on acid-triggered self-destructive and near-infrared laser light responses of nano-biocatalysts integrating starvation-chemo-photothermal therapies

作者全名："Liu, Kanglin; Yan, Shaoying; Liu, Zhengshu; Wang, Dan; Yang, Qing; Jiang, Xinyun; Chen, Liuxian; Tang, Hua"

作者地址："[Liu, Kanglin; Wang, Dan; Jiang, Xinyun; Chen, Liuxian; Tang, Hua] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis,Minist Educ, Dept Infect Dis,Key Lab Mol Biol Infect Dis, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Yan, Shaoying] Nanchang Univ, Dept Clin Lab, Affiliated Hosp 1, Nanchang, Jiangxi, Peoples R China; [Liu, Zhengshu] Chongqing Med Univ, Dept Phys Examinat, Affiliated Hosp 1, Chongqing, Peoples R China; [Wang, Dan; Yang, Qing] Peoples Hosp Rongchang Dist, Dept Pathol, Chongqing, Peoples R China"

通信作者："Tang, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis,Minist Educ, Dept Infect Dis,Key Lab Mol Biol Infect Dis, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：CANCER NANOTECHNOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000794916300001

JCR分区：Q2

影响因子：5.7

年份：2022

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Nanocatalysts; Glucose oxidase; Starvation therapy; Near-infrared laser (NIR) irradiation; Tumor therapy

摘要："Background Inherent limitations of single cancer therapy are overcome by multi-therapy modality, which integrates characteristics of each therapeutic modality and material chemistry. The multi-modal method has the potential for becoming one of the next generation options for cancer treatments. Photothermal therapy (PTT) is an efficient, non-invasive treatment method that can be used on various cancer types. We propose an acid-triggered self-destructing nano-biocatalyst integrated starvation/chemical/photothermal triple therapy that is based on design principles and biomedical applications of GOx cancer treatment methods. Methods Scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potentials were used to analyze the physical as well as chemical properties of MoS2@DOX/GOx@MnO2 (M@D/G@M). Further, Fourier transform infra-red (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) were used to assess the compositions of the nanocatalysts. The biological effects of M@D/G@M on cells were studied in vitro by inverted fluorescence microscopy, confocal laser scanning microscopy (CLSM), flow cytometry, CCK-8 test, and hemolysis test. Treatment effects of the nanocatalysts were evaluated in MHCC-97H tumor BALB/c mice, whose body weights, tumor local temperature, tumor volumes, and tumor histological changes were evaluated. Results There was a high DOX encapsulation efficiency of M@D/G@M (90.233%). The photothermal conversion efficiency (eta) of M@D/G@M is 25.2%, and its oxygen production within 5 min reached 27.5 mg L-1. Cell internalization analysis showed that within 4 h, M@D/G@M was almost completely absorbed by HepG2 cells. Further, the highest red fluorescence and apoptosis effects of dead cells (59.07% apoptosis) as well as the lowest tumor volume index of mice (0.2862%) were observed in the M@D/G@M + pH6.0 + NIR treatment group. Conclusions Our findings inform the development and applications of multi-modal methods in tumor therapy."

基金机构："key Laboratory of Infectious Diseases, CQMU [202004]; Province Natural Science Foundation of Jiangxi Province [20202BABL216040]"

基金资助正文："This study was supported by the key Laboratory of Infectious Diseases, CQMU (202004) and the Province Natural Science Foundation of Jiangxi Province (20202BABL216040)."