Biofabricated macrophage and fibroblast membranes synergistically promote skin wound healing

作者全名："Wang, Dongqing; Chen, Heying; Lei, Li; Chen, Jun; Gao, Jimin; Liu, Jiahe; Li, Qianyin; Xie, Yajun; Hu, Yi; Ni, Yilu"

作者地址："[Wang, Dongqing; Chen, Heying; Lei, Li; Liu, Jiahe; Li, Qianyin; Xie, Yajun; Ni, Yilu] Chongqing Med Univ, MOE Key Lab Lab Med Diagnost, Coll Lab Med, Chongqing, Peoples R China; [Chen, Jun; Hu, Yi] Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing, Peoples R China; [Chen, Jun; Hu, Yi] Univ Chinese Acad Sci UCAS, Chinese Acad Sci CAS, Beijing 100049, Peoples R China; [Gao, Jimin] Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Technol & Applicat Model Or, Wenzhou, Zhejiang, Peoples R China"

通信作者："Hu, Y (通讯作者)，Univ Chinese Acad Sci UCAS, Chinese Acad Sci CAS, Beijing 100049, Peoples R China.; Ni, YL (通讯作者)，Chongqing Med Univ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China.; Hu, Y (通讯作者)，Inst High Energy Phys, Beijing 100049, Peoples R China."

来源：BIOENGINEERING & TRANSLATIONAL MEDICINE

ESI学科分类：Multidisciplinary

WOS号：WOS:000805364800001

JCR分区：Q1

影响因子：7.4

年份：2022

卷号：7

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：cytomembrane; fibroblast; macrophage; wound healing

摘要："Effective skin wound healing is a complex process involving anti-inflammation, fibrosis, matrix reconstruction, and angiogenesis. This work aimed to integrate the macrophage-mediated anti-inflammation and fibroblast-assisted matrix reconstruction for enhanced skin wound healing. Herein, we utilized the cytomembranes derived from repolarized M2 macrophages and fibroblasts to prepare the natural biologics. Results showed that the inflammatory M1 macrophages were repolarized to M2 phenotype by the M2 macrophage cytomembranes. As a consequence, the cytomembranes of M2 macrophage could facilitate the wound closure in mice. Furthermore, the addition of fibroblast membranes to the macrophage cytomembranes contributed to a better matrix reconstruction, neovascularization and angiogenesis. Next, we used a transforming growth factor-beta (TGF-beta) inhibitor to attenuate cutaneous scar formation. Therefore, our modality could promote skin wound healing and effectively suppress scar formation in the preclinical murine skin wounds. The cytomembrane biologics might provide a biocompatible and versatile tool for wound healing."

基金机构："National Natural Science Foundation of China [11875269]; Natural Science Foundation of Chongqing [cstc2019jcyj-bshX0108]; Postdoctoral Innovation Program of Chongqing, China [CQBX201809]; Special Key Project of Technology Innovation and Application Development of Chongqing, China [cstc2019jscxdxwtBX0032]"

基金资助正文："National Natural Science Foundation of China, Grant/Award Number: 11875269; Natural Science Foundation of Chongqing, Grant/Award Number: cstc2019jcyj-bshX0108; Postdoctoral Innovation Program of Chongqing, China, Grant/Award Number: CQBX201809; Special Key Project of Technology Innovation and Application Development of Chongqing, China, Grant/Award Number: cstc2019jscxdxwtBX0032"