A label-free electrochemical immunosensor based on PdPtCu@BP bilayer nanosheets for point-of-care kidney injury molecule-1 testing

作者全名："Yin, Zhigang; Liu, Changjin; Yi, Yuanjie; Wu, Haiping; Fu, Xuhuai; Yan, Yurong"

作者地址："[Yin, Zhigang; Liu, Changjin; Wu, Haiping; Fu, Xuhuai; Yan, Yurong] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Yin, Zhigang; Yi, Yuanjie] Peoples Hosp Yuechi Cty, Dept Lab Med, Guanga 638000, Peoples R China; [Liu, Changjin] Zunyi Med Univ, Affiliated Hosp, Dept Lab Med, Zunyi 563003, Peoples R China"

通信作者："Yan, YR (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：JOURNAL OF ELECTROANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000807740500005

JCR分区：Q1

影响因子：4.5

年份：2022

卷号：917

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Label-free; Electrochemical immunosensor; PdPtCu@black phosphorus bilayer nanosheets; Point-of-care testing; Acute kidney injury; KIM-1

摘要："Kidney injury molecule-1 (KIM-1) protein is a vital biomarker for the early diagnosis of acute kidney injury (AKI). Therefore, a rapid and sensitive method for KIM-1 detection is urgently needed. Herein, a novel label-free electrochemical immunosensor constructed on PdPtCu@black phosphorus (BP) bilayer nanosheets (BNSs) is reported for point-of-care (POC) testing of KIM-1. Monolayer-monolayer PdPtCu@BP BNSs, a novel signal substrate, were successfully synthesized using BP nanosheets combined with trimetal PdPtCu nanosheets. Benefiting from the excellent conductivity and peroxidase-like performance of PdPtCu@BP BNSs, the designed label-free electrochemical immunosensor exhibited exceptional detection properties. Under optimal conditions, the facile immunosensor displayed satisfactory linearity from 100 pg/mL to 100 ng/mL with a minimum detection limit of 32 pg/mL. Moreover, it takes 60 min from target addition to measurement completion. Meanwhile, the developed sensing strategy was successfully applied to specifically detect KIM-1 in real samples. The proposed strategy enables rapid and sensitive protein assays, providing a potential versatile platform for POC testing."

基金机构：National Natural Science Foundation of China [81873980]; Science and Technology Project of Guizhou [Qiankehejichu-ZK (2022) 654]; Science and Technology Fund of Guizhou Health Commission [gzwkj2022-175]; Science and Technology Program of Zunyi [Zunshikeheshezi [2018] 103]; Chongqing Medical University Graduate Talent Training Program [BJRC202008]

基金资助正文："This work was funded by the National Natural Science Foundation of China (No. 81873980) , Science and Technology Project of Guizhou (No. Qiankehejichu-ZK (2022) 654) , Science and Technology Fund of Guizhou Health Commission (No. gzwkj2022-175) , Science and Technology Program of Zunyi (No. Zunshikeheshezi [2018] 103) , and Chongqing Medical University Graduate Talent Training Program (No. BJRC202008) ."