Reversely immortalized mouse salivary gland cells presented a promising metabolic and fibrotic response upon BMP9/Gdf2 stimulation

作者全名："Luo, Wenping; Liang, Panpan; Zhao, Tianyu; Cheng, Qianyu; Liu, Huikai; He, Liwen; Zhang, Linghuan; Huang, Bo; Zhang, Yuxin; He, Tongchuan; Yang, Deqin"

作者地址："[Luo, Wenping; Liang, Panpan; He, Liwen; Yang, Deqin] Chongqing Key Lab Oral Dis & Biomed Sci, 426 Songshi North Rd, Chongqing 401147, Peoples R China; [Luo, Wenping; He, Liwen; He, Tongchuan] Univ Chicago, Med Ctr, Sect Plast Surg, Dept Surg,Lab Craniofacial Biol & Dev, 5841 South Maryland Ave MC6035, Chicago, IL 60637 USA; [Liang, Panpan; Zhao, Tianyu; Cheng, Qianyu; Liu, Huikai; Zhang, Yuxin; Yang, Deqin] Chongqing Med Univ, Stomatol Hosp, 426 Songshi North Rd, Chongqing 401147, Peoples R China; [Zhao, Tianyu] Chongqing Municipal Key Lab Oral Biomed Engn High, 426 Songshi North Rd, Chongqing 401147, Peoples R China; [Zhang, Linghuan] Chongqing Med Univ, Childrens Hosp, Key Lab Child Dev & Disorders, Stem Cell Biol & Therapy Lab,Minist Educ, Chongqing 400014, Peoples R China; [Huang, Bo] Nanchang Univ, Affiliated Hosp 2, Dept Clin Lab, Jiangxi Prov Key Lab Lab Med, 1 Min De Rd, Nanchang 330006, Jiangxi, Peoples R China"

通信作者："Yang, DQ (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, 426 Songshi North Rd, Chongqing 401147, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Med Univ, Stomatol Hosp, 426 Songshi North Rd, Chongqing 401147, Peoples R China."

来源：CELLULAR & MOLECULAR BIOLOGY LETTERS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000809568300001

JCR分区：Q1

影响因子：8.3

年份：2022

卷号：27

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Submandibular gland; Sublingual gland; Immortalization; Metabolism; Fibrosis; BMP9; Gdf2

摘要："The submandibular gland (SMG) and the sublingual gland (SLG) are two of the three major salivary glands in mammals. In mice, they are adjacent to each other and open into the oral cavity, producing saliva to lubricate the mouth and aid in food digestion. Though salivary gland dysfunction accompanied with fibrosis and metabolic disturbance is common in clinic, in-depth mechanistic research is lacking. Currently, research on how to rescue salivary function is challenging, as it must resort to using terminally differentiated acinar cells or precursor acinar cells with unknown differentiation. In this study, we established reversely immortalized mouse primary SMG cells (iSMGCs) and SLG cells (iSLGCs) on the first postnatal day (P0). The iSMGCs and iSLGCs grew well, exhibited many salivary gland characteristics, and retained the metabolism-related genes derived from the original tissue as demonstrated using transcriptome sequencing (RNA-seq) analysis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of these two cell lines, which overlapped with those of the SMG and SLG, were enriched in cysteine and methionine metabolism. Furthermore, we investigated the role of bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2(Gdf2), on metabolic and fibrotic functions in the SMG and SLG. We demonstrated that iSMGCs and iSLGCs presented promising adipogenic and fibrotic responses upon BMP9/Gdf2 stimulation. Thus, our findings indicate that iSMGCs and iSLGCs faithfully reproduce characteristics of SMG and SLG cells and present a promising prospect for use in future study of salivary gland metabolism and fibrosis upon BMP9/Gdf2 stimulation."

基金机构："Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN201800436, KJZD-K201900402]; Chongqing Municipal Commission on Science Technology [cstc2017jcyjAX0434]; National Natural Science Foundation of China [31301221, 32070539, 31970783, 31571510]"

基金资助正文："The reported work was supported in part by research grants from the Project Supported by Scientific and Technological Research Program of Chongqing Municipal Education Commission (#KJQN201800436 to WL, #KJZD-K201900402 to TZ), Chongqing Municipal Commission on Science & Technology (#cstc2017jcyjAX0434 to WL), and the National Natural Science Foundation of China (No. 31301221 to WL, No. 32070539 to WL, No.31970783 to DY, and No. 31571510 to TZ)."