Interleukin 7 inhibit autophagy via P53 regulated AMPK/mTOR signaling pathway in non-small cell lung cancer

作者全名："Zhu, Yunjia; Jiang, Xi; Ding, Zhiying; Ming, Jian"

作者地址："[Zhu, Yunjia] Chongqing Med Univ, Dept Pathol, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing 400010, Peoples R China; [Jiang, Xi; Ming, Jian] Gen Hosp Northern Theater Command, Dept Pathol, 83 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China; [Ding, Zhiying] Tianjin Third Cent Hosp, Dept Pathol, 83 Jintang Rd, Tianjin 300000, Peoples R China"

通信作者："Ming, J (通讯作者)，Gen Hosp Northern Theater Command, Dept Pathol, 83 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:000819858600024

JCR分区：Q2

影响因子：4.6

年份：2022

卷号：12

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Interleukin 7 (IL-7) has been demonstrated regulating lymphangiogenesis, apoptosis, and proliferation. Whether IL-7 induce or inhibit autophagy in non-small cell lung cancer (NSCLC) are unknown. In this study, Western blot was used to detect cytoplasmic and nuclear protein of p53, total protein of AMP-activated protein kinase (AMPK), mammalian target of rapamycin (mTOR) and Light chain 3 (LC3). Quantitative Real-Time PCR (qRT-PCR) was used to detect p53 mRNA level after treated with IL-7. Then using transmission electron microscopy to observe the morphological change of autophagosome. 123 cases of NSCLC were collected for survival analysis, immunohistochemistry staining and cox regression multivariate analysis. We find that IL-7 induce the p53 translocation from nucleus to cytoplasm, then IL-7 down-regulate phosphorylation of AMPK and up-regulate phosphorylation of mTOR. The expression of AMPK and p53 were associated with IL-7/IL-7R and mTOR expression. Clinically, AMPK and p53 were well correlated with stage and survival of lung cancer patients. IL-7R, mTOR and tumor stage were the strongest predictors of survival. In conclusion, IL-7 inhibit autophagy in NSCLC via P53 regulated AMPK/mTOR signaling pathway. AMPK and p53 are correlated with patients' survival. IL-7R, mTOR and tumor stage are the strongest predictor of survival."

基金机构："National Natural Science Foundation of China [81602019]; Department of Science and Technology of Liaoning Province [2020JH2/10300166, 20170540908]"

基金资助正文："This study was supported by National Natural Science Foundation of China (Grant No: 81602019), Department of Science and Technology of Liaoning Province (Grant No. 2020JH2/10300166) and (Grant No: 20170540908)."