Multi-omics data reveals the disturbance of glycerophospholipid metabolism caused by disordered gut microbiota in depressed mice

作者全名："Tian, Tian; Mao, Qiang; Xie, Jing; Wang, Ying; Shao, Wei-hua; Zhong, Qi; Chen, Jian-jun"

作者地址："[Tian, Tian] Affiliated Hosp Guizhou Med Univ, Dept Neurol, Guiyang 550004, Guizhou, Peoples R China; [Mao, Qiang] Chongqing Med Univ, Affiliated Hosp 2, Dept Pharm, Chongqing 400010, Peoples R China; [Xie, Jing] Chongqing Univ, Peoples Hosp Chongqing 4, Cent Hosp, Chongqing Emergency Med Ctr,Dept Endocrinol, Chongqing 400016, Peoples R China; [Wang, Ying] Chongqing Med Univ, Affiliated Hosp 2, Dept Rehabil, Chongqing 400000, Peoples R China; [Shao, Wei-hua] Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing, Peoples R China; [Zhong, Qi; Chen, Jian-jun] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [Chen, Jian-jun] Chongqing Med Univ, Inst Life Sci, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Chen, JJ (通讯作者)，Chongqing Med Univ, Inst Life Sci, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：JOURNAL OF ADVANCED RESEARCH

ESI学科分类：Multidisciplinary

WOS号：WOS:000822647900008

JCR分区：Q1

影响因子：10.7

年份：2022

卷号：39

期号：　

开始页：135

结束页：145

文献类型：Article

关键词：Gut microbiota; Glycerophospholipids; Neurotransmitter; Depression

摘要："Introduction: Although researchers have done intensive research on depression, its pathogenesis is still not fully explained. More and more evidence suggests that gut microbiota is closely related to the onset of depression; but its specific functional ways are not clearly identified.Objectives: The purpose of our work was to find out how the gut microbiota was involved in the onset of depression, and to identify the potential ways to link the gut and brain in mice with depressive-like behaviors (DLB).Methods: We used the chronic restraint stress (CRS)-induced depression model here. Gut microbiota compositions in fecal samples, lipid metabolism (in fecal, serum and hippocampus samples) and neurotransmitters in hippocampus samples were detected.Results: We found that the 7 of 13 differential genera that significantly correlated with DLB belonged to phylum Firmicutes. The differential lipid metabolites in fecal samples mainly belonged to glycerophospholipids (GP) and fatty acids (FA) metabolism, and three important ""metabolite type-bacterial taxa"" correlated pairs were identified: ""FA/GP-Firmicutes"", ""FA/GP-Akkermansia"", and ""FA/GPBifidobacterium"". The key differential lipid metabolites significantly correlated with DLB mainly belonged to FA and GP, and the DLB-related metagenomic genes were consistently enriched in GP metabolism and FA metabolism. Three significantly changed short-chain fatty acids (SCFAs) were significantly correlated with the majority of differential genera. Meanwhile, we found that the differential lipid metabolites in serum and hippocampus samples were mainly mapped into the GP metabolism, and there were four differential neurotransmitters from the tryptophan pathway in hippocampus samples.Conclusion: Together, our findings could provide novel insights into the role of ""microbiota-gut-brain"" (MGB) axis in depression, and indicate that the gut microbiota might have a vital role in the onset of DLB by affecting the peripheral/central GP metabolism and tryptophan pathway. The ""FirmicutesSCFAs-GP metabolism-Tryptophan pathway"" might be a possible way to link the gut and brain in depressed mice.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)."

基金机构："Natural Science Foundation Project of China [81701360, 82160273]; Natural Science Foun-dation of Chongqing, China [cstc2021jcyj-msxmX0084]; Natural Science Foundation of Guizhou Province, China; Science and Technology Research Program of Chongqing Municipal Education Commission, China [KJQN202100420]; Chongq-ing Yuzhong District Science & Technology Commission, China [20190115]"

基金资助正文："This work was supported by the Natural Science Foundation Project of China (81701360, 82160273) , the Natural Science Foundation of Chongqing, China (cstc2021jcyj-msxmX0084) , the Natural Science Foundation of Guizhou Province, China (Grant No. Qianke He Foundation-ZK [2021] General 416) , the Science and Technology Research Program of Chongqing Municipal Education Commission, China (Grant No. KJQN202100420) and the Chongqing Yuzhong District Science & Technology Commission, China (20190115) . The 16s data analysis and metagenomic analysis were performed using the free online platform of Majorbio Cloud Plat-form, and the metabolites data were detected in Shanghai Bio-clouds Biotechnology Co., Ltd."