RBM47 inhibits hepatocellular carcinoma progression by targeting UPF1 as a DNA/RNA regulator

作者全名："Guo, Tao; You, Ke; Chen, Xi; Sun, Yuqi; Wu, Ying; Wu, Ping; Jiang, Yingying"

作者地址："[Guo, Tao] Weifang Med Univ, Sch Basic Med Sci, Dept Pathophysiol, Weifang 261053, Peoples R China; [You, Ke] Chongqing Med Univ, Affiliated Hosp 2, Dept Hepatobiliary Surg, Chongqing 400010, Peoples R China; [Chen, Xi; Jiang, Yingying] Weifang Med Univ, Sch Stomatol, Weifang 261053, Peoples R China; [Sun, Yuqi] Weifang Med Univ, Sch Clin Med, Weifang 261031, Peoples R China; [Wu, Ying] Guangxi Med Univ, Liuzhou Peoples Hosp, Key Lab Clin Biotechnol, Liuzhou Key Lab Infect Dis Immun Res,Guangxi Hlth, Liuzhou 545006, Peoples R China; [Wu, Ping] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangzhou Inst Pediat, Dept Pediat Surg, Guangzhou 510000, Peoples R China; [Jiang, Yingying] Weifang Med Univ, Affiliated Hosp, Dept Dent, Weifang 261035, Peoples R China"

通信作者："Jiang, YY (通讯作者)，Weifang Med Univ, Sch Stomatol, Weifang 261053, Peoples R China.; Jiang, YY (通讯作者)，Weifang Med Univ, Affiliated Hosp, Dept Dent, Weifang 261035, Peoples R China."

来源：CELL DEATH DISCOVERY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000824635200001

JCR分区：Q2

影响因子：7

年份：2022

卷号：8

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："The mechanisms by which the tumor behaviors of hepatocellular carcinoma (HCC) support growth and metastasis remain largely unknown, and it has become increasingly apparent that molecular dysregulation is of considerable importance for cellular signaling pathways. Recently, RNA-binding motif protein 47 (RBM47) has been suggested to function as a tumor regulator by acting as an RNA binding protein (RBP), but its role in HCC remains ambiguous. Here, in HCC, we identified that RBM47 had an inhibitory influence on tumor behaviors in vitro and accordingly suppressed the growth and metastasis of xenograft tumors in vivo. Additionally, RBM47 was verified to positively regulate Upframeshift 1 (UPF1), which is a crucial protein involved in the nonsense-mediated RNA decay (NMD) process and was previously determined to be an HCC suppressor. Mechanistically, the stability of UPF1 mRNA was demonstrated to be enhanced with its 3'UTR bound by RBM47, which acted as an RNA binding protein. Meanwhile, RBM47 was also proven to promote the transcription of UPF1 as a transcription factor. Taken together, we concluded that RBM47 functioned as a tumor suppressor by upregulating UPF1, acting as a DNA/RNA binding protein at the transcriptional and posttranscriptional levels."

基金机构："National Natural Science Foundation of China [82103166]; Natural Science Foundation of Shandong Province China [ZR2021QH200, ZR2020MH192]; 2021 Youth Innovation Talent Introduction and Education Program of Shandong Province Universities"

基金资助正文："This work was supported by the National Natural Science Foundation of China (82103166), the Natural Science Foundation of Shandong Province China (ZR2021QH200 and ZR2020MH192), and 2021 Youth Innovation Talent Introduction and Education Program of Shandong Province Universities (Yingying Jiang)."