"Programmable, Universal DNAzyme Amplifier Supporting Pancreatic Cancer-Related miRNAs Detection"

作者全名："Nie, Kunhan; Jiang, Yongjian; Wang, Na; Wang, Yajun; Li, Di; Zhan, Lei; Huang, Chengzhi; Li, Chunmei"

作者地址："[Nie, Kunhan; Jiang, Yongjian; Wang, Na; Wang, Yajun; Zhan, Lei; Huang, Chengzhi; Li, Chunmei] Southwest Univ, Coll Pharmaceut Sci, Minist Educ, Key Lab Luminescence Anal & Mol Sensing, Chongqing 400715, Peoples R China; [Li, Di] Chongqing Med Univ, Dept Pharm, Affiliated Hosp 2, Chongqing 400010, Peoples R China"

通信作者："Li, CM (通讯作者)，Southwest Univ, Coll Pharmaceut Sci, Minist Educ, Key Lab Luminescence Anal & Mol Sensing, Chongqing 400715, Peoples R China."

来源：CHEMOSENSORS

ESI学科分类：　

WOS号：WOS:000831902100001

JCR分区：Q2

影响因子：4.2

年份：2022

卷号：10

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：DNAzyme amplifier; signal amplification; miRNA detection; pancreatic cancer

摘要："The abnormal expression of miRNA is closely related to the occurrence of pancreatic cancer. Herein, a programmable DNAzyme amplifier for the universal detection of pancreatic cancer-related miRNAs was proposed based on its programmability through the rational design of sequences. The fluorescence signal recovery of the DNAzyme amplifier showed a good linear relationship with the concentration of miR-10b in the range of 10-60 nM, with a detection limit of 893 pM. At the same time, this method displayed a high selectivity for miR-10b, with a remarkable discrimination of a single nucleotide difference. Furthermore, this method was also successfully used to detect miR-21 in the range of 10-60 nM based on the programmability of the DNA amplifier, exhibiting the universal application feasibility of this design. Overall, the proposed programmable DNAzyme cycle amplifier strategy shows promising potential for the simple, rapid, and universal detection of pancreatic cancer-related miRNAs, which is significant for improving the accuracy of pancreatic cancer diagnosis."

基金机构：National Natural Science Foundation of China (NSFC) [22074124]; fund of Fundamental Research Funds for the Central Universities [XDJK2020TY001]; Chongqing Talents Program for Outstanding Scientists [cstc2021ycjh-bgzxm0179]; Chongqing Graduate Student Scientific Research Innovation Project [CYB21119]

基金资助正文："This research was funded by the National Natural Science Foundation of China (NSFC, no. 22074124), the fund of Fundamental Research Funds for the Central Universities (XDJK2020TY001), Chongqing Talents Program for Outstanding Scientists (No. cstc2021ycjh-bgzxm0179), and the Chongqing Graduate Student Scientific Research Innovation Project (CYB21119)."