ERK1/2 Signalling Pathway Regulates Tubulin-Binding Cofactor B Expression and Affects Astrocyte Process Formation after Acute Foetal Alcohol Exposure

作者全名："Zheng, Yin; Huo, Jiechao; Yang, Mei; Zhang, Gaoli; Wan, Shanshan; Chen, Xiaoqiao; Zhang, Bingqiu; Liu, Hui"

作者地址："[Zheng, Yin; Huo, Jiechao; Yang, Mei; Chen, Xiaoqiao; Zhang, Bingqiu; Liu, Hui] Chongqing Med Univ, Inst Neurosci, Chongqing 400016, Peoples R China; [Zhang, Gaoli] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Chongqing 400063, Peoples R China; [Wan, Shanshan] Sichuan Canc Hosp & Inst, Dept Blood Transfus, Chengdu 610044, Peoples R China"

通信作者："Zheng, Y; Huo, JC (通讯作者)，Chongqing Med Univ, Inst Neurosci, Chongqing 400016, Peoples R China."

来源：BRAIN SCIENCES

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000832315700001

JCR分区：Q3

影响因子：3.3

年份：2022

卷号：12

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：tubulin-binding cofactor B; astrocyte processes; microtubules; extracellular signal-regulated protease 1/2 signalling pathway; foetal alcohol spectrum disorders; acute foetal alcohol exposure

摘要："Foetal alcohol spectrum disorders (FASDs) are a spectrum of neurological disorders whose neurological symptoms, besides the neuronal damage caused by alcohol, may also be associated with neuroglial damage. Tubulin-binding cofactor B (TBCB) may be involved in the pathogenesis of FASD. To understand the mechanism and provide new insights into the pathogenesis of FASD, acute foetal alcohol exposure model on astrocytes was established and the interference experiments were carried out. First, after alcohol exposure, the nascent astrocyte processes were reduced or lost, accompanied by the absence of TBCB expression and the disruption of microtubules (MTs) in processes. Subsequently, TBCB was silenced with siRNA. It was severely reduced or lost in nascent astrocyte processes, with a dramatic reduction in astrocyte processes, indicating that TBCB plays a vital role in astrocyte process formation. Finally, the regulating mechanism was studied and it was found that the extracellular signal-regulated protease 1/2 (ERK1/2) signalling pathway was one of the main pathways regulating TBCB expression in astrocytes after alcohol injury. In summary, after acute foetal alcohol exposure, the decreased TBCB in nascent astrocyte processes, regulated by the ERK1/2 signalling pathway, was the main factor leading to the disorder of astrocyte process formation, which could contribute to the neurological symptoms of FASD."

基金机构："National Natural Science Foundation of China [81971230, 81500978, 81671312, 81000566, cstc2016jcyjA0229, cstc2017jcyjAX0414, cstc2015jcyja10018, cstc2011jjA10093]; Foundation of Chongqing Municipal Education Commission [KJ1600213]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (81971230, 81500978, 81671312, 81000566), theNatural Science Foundation Project of Chong Qing (cstc2016jcyjA0229, cstc2017jcyjAX0414, cstc2015jcyja10018, cstc2011jjA10093), and the Foundation of Chongqing Municipal Education Commission (KJ1600213)."