A Cross-Sectional Study Revealing the Emergence of Erythromycin-Resistant Bordetella pertussis Carrying ptxP3 Alleles in China

作者全名："Wu, Xiaoying; Du, Qianqian; Li, Dongfang; Yuan, Lin; Meng, Qinghong; Fu, Zhou; Xu, Hongmei; Yao, Kaihu; Zhao, Ruiqiu"

作者地址："[Wu, Xiaoying; Xu, Hongmei; Zhao, Ruiqiu] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Dept Infect Dis,Minist Educ,Key Lab Child Dev & Di, Chongqing, Peoples R China; [Du, Qianqian; Yuan, Lin; Meng, Qinghong; Yao, Kaihu] Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Natl Ctr Childrens Hlth,Beijing Key Lab Pediat Res, Beijing, Peoples R China; [Li, Dongfang] BGI Pathogenesis Pharmaceut Technol, BGI Shenzhen, Shenzhen, Peoples R China; [Fu, Zhou] Childrens Hosp Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Resp Dis, Minist Educ Key Lab Child Dev & Disorders,Chongqin, Chongqing, Peoples R China"

通信作者："Zhao, RQ (通讯作者)，Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Dept Infect Dis,Minist Educ,Key Lab Child Dev & Di, Chongqing, Peoples R China.; Yao, KH (通讯作者)，Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Natl Ctr Childrens Hlth,Beijing Key Lab Pediat Res, Beijing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000834548100001

JCR分区：Q2

影响因子：5.2

年份：2022

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Bordetella pertussis; virulence-related genotypes; erythromycin-resistance; genome sequence; ptxp3; molecular epidemiology

摘要："BackgroundPrevious limited studies have identified that Bordetella pertussis (B. pertussis) isolates circulating in China possess distinct molecular features and high rates of erythromycin-resistance (ER). Their evolution and potential impact on the prevention and control of global pertussis are worthy of attention. MethodsThe present cross-sectional study involved 311 non-duplicate and unrelated B. pertussis strains isolated from Chinese children from 2017 to 2019. Their antimicrobial susceptibilities were assessed using both E-test strips and Kirby-Bauer (KB) disk diffusion methods. Seven virulence-related genes (ptxA, ptxC, ptxP, prn, fim2, fim3, and tcfA2) and the A2047G mutation in the 23S rRNA gene were detected by PCR. Based on the susceptibilities and genotypes, 50 isolates were selected for multi-locus variable-number tandem-repeat analysis (MLVA) typing and whole-genome sequencing. ResultsA total of 311 B. pertussis strains were isolated from children with a median age of 4 months (interquartile range: 2-9 months). Strains carrying the ptxP1 allele were more frequent (84.9%, 264/311), were always ER (except for one strain), and were mainly related to ptxA1/ptxC1/prn1 alleles (99.6%, 263/264). The remaining 47 (15.1%) strains carried the ptxP3 allele, mainly harboring the ptxA1/ptxC2/prn2 alleles (93.6%, 44/47), and were sensitive to erythromycin (except for two strains). The two ER-ptxP3 isolates were first identified in China, belonged to MT27 and MT28 according to MLVA, and were classified into sub-lineage IVd by phylogenetic analysis of their genome sequences. This sub-lineage also includes many strains carrying the ptxP3 allele spreading in developed countries. For each tested antimicrobial, the susceptibilities judged by KB disks were consistent with those determined by E-test strips. ConclusionThe present results reveal that B. pertussis strains with the ptxP1-ER profile still dominate in China, and a few strains carrying the ptxP3 allele have acquired the A2047G mutation in the 23S rRNA gene and the ER phenotype. The surveillance of the drug susceptibility of B. pertussis is necessary for all countries, and the KB disk method can be adopted as a screening test."

基金机构："National Natural Science Foundation of China [81973100]; Natural Science Foundation of Beijing Haidian Original Innovation Fund Joint Project [19L2041]; MOE Key Laboratory of Major Diseases in Children in Beijing Childrens Hospital, Capital Medical University [2021-03]"

基金资助正文："This work was supported by the National Natural Science Foundation of China [81973100], the Natural Science Foundation of Beijing Haidian Original Innovation Fund Joint Project [19L2041], and an open research topic in MOE Key Laboratory of Major Diseases in Children in Beijing Childrens Hospital, Capital Medical University [2021-03]."