MicroRNA-29b/graphene oxide-polyethyleneglycol-polyethylenimine complex incorporated within chitosan hydrogel promotes osteogenesis

作者全名："Qin, Han; Ji, Yujie; Li, Guangyue; Xu, Xiaohui; Zhang, Chuangwei; Zhong, Wenjie; Xu, Shihan; Yin, Yuanyuan; Song, Jinlin"

作者地址："[Qin, Han; Ji, Yujie; Li, Guangyue; Xu, Xiaohui; Zhang, Chuangwei; Zhong, Wenjie; Xu, Shihan; Yin, Yuanyuan; Song, Jinlin] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Qin, Han; Ji, Yujie; Li, Guangyue; Xu, Xiaohui; Zhang, Chuangwei; Zhong, Wenjie; Xu, Shihan; Yin, Yuanyuan; Song, Jinlin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Qin, Han; Ji, Yujie; Li, Guangyue; Xu, Xiaohui; Zhang, Chuangwei; Zhong, Wenjie; Xu, Shihan; Yin, Yuanyuan; Song, Jinlin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Song, JL (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China.; Song, JL (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Song, JL (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：FRONTIERS IN CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000837293300001

JCR分区：Q2

影响因子：5.5

年份：2022

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：graphene oxide; gene delivery system; miR-29b; functionalization; osteogenesis; chitosan hydrogel; polyethylenimine; polyethyleneglycol

摘要："MicroRNAs (miRNAs) play a pivotal role in regulating a number of physiologic and pathologic processes including bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation, making them a candidate used to promote osteogenesis. However, due to intrinsic structure and characteristics, ""naked "" miRNAs are unstable in serum and could not pass across the cellular membrane. Nano delivery systems seem to be a solution to these issues. Recently, graphene oxide (GO)-based nanomaterials are considered to be promising for gene delivery due to their unique physiochemical characteristics such as high surface area, biocompatibility, and easy modification. In this work, a GO-based nanocomplex functionalized by polyethyleneglycol (PEG) and polyethylenimine (PEI) was prepared for loading and delivering miR-29b, which participates in multiple steps of bone formation. The nanocomplex revealed good biocompatibility, miRNA loading capacity, and transfection efficiency. The miR-29b/GO-PEG-PEI nanocomplex was capsulated into chitosan (CS) hydrogel for osteogenesis. In vitro and in vivo evaluation indicated that miR-29b/GO-PEG-PEI@CS composite hydrogel was able to promote BMSC osteogenic differentiation and bone regeneration. All these results indicate that PEG/PEI functionalized GO could serve as a promising candidate for miRNA cellular delivery, and the miR-29b/GO-PEG-PEI@CS hydrogel has the potential for repairing bone defects in vivo."

基金机构："National Natural Science Foundation of China [82001063, 31971282]; Post-doctoral Program of Natural Science Foundation of Chongqing [cstc2020jcyj-bshX0108]; Program for Innovation Team Building at Institutions of Higher Education in Chongqing [CXTDG201602006]; Program for Team Building of Research Supervisor in Chongqing [dstd201903]"

基金资助正文："All authors gave final approval and agreed to be accountable for all aspects of the work. This work was supported by the National Natural Science Foundation of China (Grant No. 82001063 and 31971282), Post-doctoral Program of Natural Science Foundation of Chongqing (Grant No. cstc2020jcyj-bshX0108), Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016 (Grant No. CXTDG201602006), and Program for Team Building of Research Supervisor in Chongqing in 2019 (Grant No. dstd201903)."