Synergistic effect of VEGF and SDF-1 alpha in endothelial progenitor cells and vascular smooth muscle cells

作者全名："Yang, Haiyan; He, Cancan; Bi, Yang; Zhu, Xu; Deng, Dan; Ran, Tingting; Ji, Xiaojuan"

作者地址："[Yang, Haiyan; Ji, Xiaojuan] Chongqing Gen Hosp, Dept Ultrasound, Chongqing, Peoples R China; [Yang, Haiyan] Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai, Peoples R China; [He, Cancan] Affiliated Hosp Zunyi Med Univ, Guizhou Childrens Hosp, Dept Pediat, Zunyi, GZ, Peoples R China; [Bi, Yang; Zhu, Xu; Ran, Tingting] Chongqing Med Univ, Natl Clin & Res Ctr Child Hlth & Disorders, Chongqing Engn Res Ctr Stem Cell Therapy, Dept Ultrasound,Childrens Hosp,Minist Educ Key Lab, Chongqing, Peoples R China; [Deng, Dan] Changsha Med Univ, Sch Med Imaging, Changsha, Peoples R China"

通信作者："Ji, XJ (通讯作者)，Chongqing Gen Hosp, Dept Ultrasound, Chongqing, Peoples R China."

来源：FRONTIERS IN PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000841764100001

JCR分区：Q1

影响因子：5.6

年份：2022

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：vascular smooth muscle cells; vascular endothelial growth factor; endothelial progenitor cell; stromal cell-derived factor-1 alpha; synergistic effect

摘要："Vascular endothelial growth factor (VEGF) is a potent agonist of angiogenesis that induces proliferation and differentiation of endothelial progenitor cells (EPCs) after vascular injury. Previous studies have suggested that stromal cell-derived factor 1-alpha (SDF-1 alpha) and VEGF have a synergistic effect on vascular stenosis. The aim of the present study was to investigate whether VEGF and SDF-1 alpha act synergistically in EPCs and vascular smooth muscle cells (VSMCs). In this study, EPCs were isolated from rat bone marrow and their morphology and function were studied. Subsequently, VEGF was delivered into EPCs using an adenoviral vector. Tube formation, migration, proliferation, and apoptosis of VEGF-overexpressing EPCs was analyzed. Then, EPCs were co-cultured with VSMCs in the presence or absence of SDF-1 alpha, the migration, proliferation, apoptosis, and differentiation capacity of EPCs and VSMCs were analyzed respectively. The isolated EPCs showed typical morphological features, phagocytic capacity, and expressed surface proteins. While stable expression of VEGF remarkably enhanced tube formation, migration, and proliferation capacity of EPCs, apoptosis was decreased. Moreover, the proliferation, migration, and differentiation capacity of EPCs in the co-cultured model was enhanced in the presence of SDF-1 alpha, and apoptosis was decreased. However, these effects were reversed in VSMCs. Therefore, our results showed that VEGF and SDF-1 alpha synergistically increased the migration, differentiation, and proliferation capabilities of EPCs, but not VSMCs. This study suggests a promising strategy to prevent vascular stenosis."

基金机构：Project of Chongqing Municipal Human Resources and Social Security Bureau; [cx2019065]

基金资助正文：Funding This work was supported by Project of Chongqing Municipal Human Resources and Social Security Bureau (cx2019065).