The PNA mouse may be the best animal model of polycystic ovary syndrome

作者全名："Ren, Jingyi; Tan, Guangqing; Ren, Xinyi; Lu, Weiyu; Peng, Qiling; Tang, Jing; Wang, Yingxiong; Xie, Biao; Wang, Meijiao"

作者地址："[Ren, Jingyi; Tan, Guangqing; Ren, Xinyi; Lu, Weiyu; Wang, Meijiao] Chongqing Med Univ, Coll Basic Med, Dept Physiol, Chongqing, Peoples R China; [Peng, Qiling; Wang, Yingxiong] Chongqing Med Univ, Coll Basic Med, Chongqing, Peoples R China; [Tang, Jing; Wang, Yingxiong; Wang, Meijiao] Chongqing Med Univ, Coll Publ Hlth & Management, Joint Int Res Lab Reprod, Dev Minist Educ China, Chongqing, Peoples R China; [Tang, Jing] Chongqing Med Univ, Coll Basic Med, Dept Bioinformat, Chongqing, Peoples R China; [Xie, Biao] Chongqing Med Univ, Sch Publ Hlth & Management, Dept Biostat, Chongqing, Peoples R China"

通信作者："Wang, MJ (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Physiol, Chongqing, Peoples R China.; Wang, MJ (通讯作者)，Chongqing Med Univ, Coll Publ Hlth & Management, Joint Int Res Lab Reprod, Dev Minist Educ China, Chongqing, Peoples R China.; Xie, B (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Dept Biostat, Chongqing, Peoples R China."

来源：FRONTIERS IN ENDOCRINOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000843400500001

JCR分区：Q1

影响因子：5.2

年份：2022

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：polycystic ovary syndrome (PCOS); bioinformatics; animal model; dehydroepiandrosterone (DHEA); differentially expressed genes (DEG); RNA sequencing (RNA-seq)

摘要："Polycystic ovary syndrome (PCOS) exerts negative effects on females of childbearing age. It is important to identify more suitable models for fundamental research on PCOS. We evaluated animal models from a novel perspective with the aim of helping researchers select the best model for PCOS. RNA sequencing was performed to investigate the mRNA expression profiles in the ovarian tissues of mice with dehydroepiandrosterone (DHEA) plus high-fat diet (HFD)-induced PCOS. Meanwhile, 14 datasets were obtained from the Gene Expression Omnibus (GEO), including eight studies on humans, three on rats and three on mice, and genes associated with PCOS were obtained from the PCOSKB website. We compared the consistency of each animal model and human PCOS in terms of DEGs and pathway enrichment analysis results. There were 239 DEGs shared between prenatally androgenized (PNA) mice and PCOS patients. Moreover, 1113 genes associated with PCOS from the PCOSKB website were identified among the DEGs of PNA mice. A total of 134 GO and KEGG pathways were shared between PNA mice and PCOS patients. These findings suggest that the PNA mouse model is the best animal model to simulate PCOS."

基金机构：National Natural Science Foundation of China; Chongqing Natural Science Foundation; Science and Technology Project of Chongqing Yuzhong District; Scientific Research & Innovation Experiment Project of Chongqing Medical University; Chongqing Postdoctoral Science Foundation; [82171624]; [81972023]; [cstc2020jcyj-msxmX0294]; [20200103]; [SRIEP202106]; [SRIEP202002]; [cstc2021jcyj-bsh0029]

基金资助正文："Funding The study was funded by National Natural Science Foundation of China (No. 82171624; 81972023), the Chongqing Natural Science Foundation (No. cstc2020jcyj-msxmX0294), Science and Technology Project of Chongqing Yuzhong District (No. 20200103), Scientific Research & Innovation Experiment Project of Chongqing Medical University (SRIEP202106; SRIEP202002) and the Chongqing Postdoctoral Science Foundation (No. cstc2021jcyj-bsh0029)."