Gut microbiota and short-chain fatty acids may be new biomarkers for predicting neonatal necrotizing enterocolitis: A pilot study

作者全名："Liu, Xiao-Chen; Du, Ting-Ting; Gao, Xiong; Zhao, Wen-Jing; Wang, Zheng-Li; He, Yu; Bao, Lei; Li, Lu-Quan"

作者地址："[Liu, Xiao-Chen; Du, Ting-Ting; Gao, Xiong; Zhao, Wen-Jing; Wang, Zheng-Li; He, Yu; Bao, Lei; Li, Lu-Quan] Chongqing Med Univ, Neonatal Diag & Treatment Ctr, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders,Minist Ed, Chongqing, Peoples R China"

通信作者："Li, LQ (通讯作者)，Chongqing Med Univ, Neonatal Diag & Treatment Ctr, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders,Minist Ed, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000848130600001

JCR分区：Q2

影响因子：5.2

年份：2022

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：neonatal necrotizing enterocolitis; gut microbiota; short-chain fatty acids; metabolites; predict

摘要："BackgroundDysbacteriosis is thought to play an important role in the pathogenesis of necrotizing enterocolitis (NEC). We aimed to identify new biomarkers among gut microbiota and short-chain fatty acids (SCFAs) for the early prediction of NEC. Materials and methodsThirty-four preterm infants with gestational ages of <= 34 weeks who developed gastrointestinal symptoms were divided into the NEC group (n = 17) and non-NEC group (n = 17). In the NEC group, the gut microbiota and SCFAs in feces were assessed when the infants were enrolled (Group P) and when they were diagnosed with NEC (Group N). In the non-NEC group, samples were assessed when the infants were enrolled (Group C). ResultsThe Ace and Chao1 indices were higher in Group P than in Group C (P < 0.05), and there was no difference between Groups C and N or between Groups P and N (P > 0.05). There was no significant difference in the Simpson and Shannon indices among Groups C, P and N (P > 0.05). The four main phyla showed no differences (P > 0.05) in composition, while at the genus level, compared with Group C, in Group P, Clostridioides, Blautia and Clostridium_sensu_stricto_1 were increased, while Lactobacillus and Bifidobacterium were decreased (P < 0.05). At the species level, Streptococcus salivarius and Rothia mucilaginosa increased, while Bifidobacterium animals subsp. lactis decreased (P < 0.05). In Group N, at the genus level, Stenotrophomonas, Streptococcus and Prevotella increased (P < 0.05). Compared with those in Group C, the levels of acetic acid, propanoic acid and butyric acid decreased significantly in Groups P and N (P < 0.05), and the areas under the curves (AUCs) of these three SCFAs between groups C and P were 0.73, 0.70, and 0.68, respectively. ConclusionThe increase in Streptococcus salivarius and Rothia mucilaginosa and decrease in Bifidobacterium_animals_subsp._lactis, as well as the decrease in acetic, propionic and butyric acids, may help in the early prediction of NEC."

基金机构：Natural Science Foundation of Chongqing municipality [cstc2021jcyj-msxmX0063]; Joint Medical Research Project of Chongqing Science and Technology Commission [2022MSXM039]; China Postdoctoral Science Foundation [2022MD713716]; Ministry of Education Key Laboratory of Child Development and Disorders [GBRP-202107]; Science and Health Project of Chongqing Health Commission [2020FYYX217]; National Natural Science Foundation of China [82001602]

基金资助正文："This work was supported by the Natural Science Foundation of Chongqing municipality (cstc2021jcyj-msxmX0063), Joint Medical Research Project of Chongqing Science and Technology Commission (2022MSXM039), China Postdoctoral Science Foundation (No.2022MD713716), the General Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders (No. GBRP-202107), Science and Health Project of Chongqing Health Commission (2020FYYX217), and National Natural Science Foundation of China (82001602)."