Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model

作者全名："Li, Yuanyuan; Xu, Ximing; Guo, Ziyao; Li, Qinyuan; Wang, Yiying; Jian, Ding; Zhang, Guangli; Tian, Xiaoyin; Chen, Shiyi; Luo, Zhengxiu"

作者地址："[Li, Yuanyuan; Guo, Ziyao; Li, Qinyuan; Wang, Yiying; Jian, Ding; Zhang, Guangli; Tian, Xiaoyin; Chen, Shiyi; Luo, Zhengxiu] Chongqing Med Univ, Dept Resp Med Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, Chongqing, Peoples R China; [Li, Yuanyuan; Guo, Ziyao; Li, Qinyuan; Wang, Yiying; Jian, Ding; Chen, Shiyi] Chongqing Key Lab Pediat, Chongqing Key Lab Child Infect & Immun, Chongqing, Peoples R China; [Xu, Ximing] Chongqing Med Univ, Dept Med Record Stat Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, Chongqing, Peoples R China; [Xu, Ximing] Chongqing Higher Inst Engn Res Ctr, Childrens Med Big Data Intelligent Applicat, Chongqing, Peoples R China"

通信作者："Luo, ZX (通讯作者)，Chongqing Med Univ, Dept Resp Med Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000849928400001

JCR分区：Q2

影响因子：5.2

年份：2022

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：long-lasting dysbiosis; mice; gut microbiota; neonatal infection; Streptococcus pneumoniae

摘要："Early life is a ""critical window"" for gut microbiota development, antibiotic use during this period exerts a profound effect on gut microbial dysbiosis and asthma. In clinical practice, antibiotics are usually used in patients with bacterial infections, we previously showed that neonatal S. pneumoniae pneumonia promoted adult-onset asthma in mice model, while it remains unclear whether neonatal S. pneumoniae infection have long-term effects on gut microbiota. Neonatal BALB/c mice were inoculated with 5*10(6) CFU D39 to establish non-lethal S. pneumoniae pneumonia model. At 2, 3, 8 weeks of age, feces in the cecum were prepared for 16S rRNA sequencing, lungs were collected for histopathologic and lung function analysis. S. pneumoniae-infected neonatal mice exhibited histopathologic lesions in their lungs and increased airway hyperresponsiveness, obvious alterations in alpha and beta diversities in the entire gut microbiota, and changes of the community structure during the breastfeeding period, infancy, and adulthood. Furthermore, gut microbial composition was modified after neonatal S. pneumoniae infection, with a decreased relative abundance of Lactobacillus in the breastfeeding period and infancy; in adulthood, the relative abundance of Allobaculum diminished while that of Proteobacteria was augmented. Neonatal S. pneumoniae infection induced a long-term alteration in microbial community composition."

基金机构：Science and Technology Department of Chongqing; Health Committee of Chongqing [cstc2020jcyj-msxmX0677]; [CQYC2020030393]

基金资助正文：This work was supported by the Science and Technology Department of Chongqing (cstc2020jcyj-msxmX0677) and the Health Committee of Chongqing (CQYC2020030393).