Diagnosis of Alzheimer's Disease and In Situ Biological Imaging via an Activatable Near-Infrared Fluorescence Probe

作者全名："Yang, Yuexia; Zhang, Liang; Wang, Jiemin; Cao, Yuping; Li, Shuyan; Qin, Wenwu; Liu, Yun"

作者地址："[Yang, Yuexia; Wang, Jiemin; Cao, Yuping; Li, Shuyan; Qin, Wenwu] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat G, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China; [Yang, Yuexia; Wang, Jiemin; Cao, Yuping; Li, Shuyan; Qin, Wenwu] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Special Funct Mat & Struct Design, MOE, Lanzhou 730000, Peoples R China; [Zhang, Liang] Army Med Univ, Xinqiao Hosp, Dept Radiol, Chongqing 400037, Peoples R China; [Liu, Yun] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China"

通信作者："Qin, WW (通讯作者)，Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat G, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.; Qin, WW (通讯作者)，Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Special Funct Mat & Struct Design, MOE, Lanzhou 730000, Peoples R China.; Liu, Y (通讯作者)，Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000856067300001

JCR分区：Q1

影响因子：7.4

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：　

摘要："Alzheimer's disease (AD) is a common neuro-degenerative disease that makes the brain nervous system degenerate rapidly and is accompanied by some special cognitive and behavioral dysfunction. Recently, butyrylcholinesterase (BChE) was reported as an important enzyme, whose activity can provide predictive value for timely discovery and diagnosis of AD. Therefore, it is indispensable to design a detection tool for selective and rapid response toward BChE. In this study, we developed a novel near-infrared fluorescent probe (Chy-1) for the detection of BChE activity. An excellent sensitivity, good biocompatibility, and lower limit of detection (LOD) of 0.12 ng/mL made the probe extremely specific for BChE, which was successfully used in biological imaging. What is more, Chy-1 can not only clearly distinguish tumor from normal cells but also forms a clear boundary between the normal and cancer tissues due to the obvious difference in fluorescence intensity produced via in situ spraying. Most important of all, Chy-1 was also successfully applied to track the BChE activity in AD mouse models. Based on this research, the novel probe may be a powerful tool for clinical diagnosis and therapy of tumor and neurodegenerative diseases."

基金机构："National Natural Science Foundation of China [21771092, 81903561]; ""International Cooperation Program of Gansu Province"" [20YF3WA012]; Fundamental Research Funds for the Central Universities [lzujbky-2021-kb17]; Special Fund Project of Guiding Scientific and Technological Innovation Development of Gansu Province [2019ZX-04]; 111 Project [B20027]"

基金资助正文："The authors would like to thank The National Natural Science Foundation of China (nos. 21771092 and 81903561). This work was supported by the ""International Cooperation Program of Gansu Province"" (20YF3WA012), the Fundamental Research Funds for the Central Universities (lzujbky-2021-kb17), and the Special Fund Project of Guiding Scientific and Technological Innovation Develop-ment of Gansu Province (2019ZX-04). This work was also supported by the 111 Project, B20027."